MAB3560-C
Anti-8-Oxoguanine, clone 483.15 Antibody (Ascites Free)
clone 483.15, from mouse
Synonim(y):
8-Oxoguanine
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
483.15, monoclonal
reaktywność gatunkowa
rat, human, monkey, bovine, mouse
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
metody
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
izotyp
IgMκ
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... OGG1(4968)
Opis ogólny
8-Oxoguanine is a mutagenic oxidative damage product of guanine. Oxidatively damaged base 8-oxoguanine is generated in the DNA of all living organisms due to the presence of reactive oxygen species in cells. DNA damage threatens the health of the genetic information stored in every DNA molecule; however enzymes exist in cells to protect against the mutagenic effect of this lesion. 8-oxoguanine is one of the most abundant and well-characterized DNA lesions generated by oxidative stress. It has been estimated that ~180 guanines are oxidized to 8-oxoG per mammalian cell per day. 8-oxoG is a miscoding lesion that can cause G:C to T:A or T:A to G:C transversion mutations. This lesion accumulates in DNA with age and it has been loosly linked to several cancers and diseases, such as Alzheimer′s and Parkinson′s.
Specyficzność
8-oxoguanine. By competitive ELISA the monoclonal showed no reactivity with dGMP, dAMP, dCMP or TMP in micromolar concentrations. Competition was only observed when the concentrations were increased to the millimolar range.
Target DNA modification is not species-specific.
Immunogen
8-oxoguanine adsorbed on to alumina.
Zastosowanie
Immunohistochemistry Analysis: A representative lot detected cells with 8-oxoguanine (8-oxoG) immunoreactivity in the central veins (CV) and portal triads (PT) regions of paraffin-embedded kettle liver sections (García-Vaquero, M., et al. (2012). Animal.6(9):1435-1443).
Immunohistochemistry Analysis: A representative lot detected increased nuclear 8-oxoguanine (8-oxoG) immunoreactivity in paraffin-embedded coronal hippocampus sections from mice induced to express mutated mitochondrial isoform Uracil–DNA glycosylase by fluorescent immunohistochemistry (Lauritzen, K.H., et al. (2011). DNA Repair (Amst). 10(6):639-653).
Immunohistochemistry Analysis: A representative lot detected significantly increased nuclear 8-oxoguanine (8-oxoG) immunoreactivity in ovaries excised from neonatal mice upon exposure to ovotoxic agent 4-vinylcyclohexene diepoxide (VCD), methoxychlor (MXC), or menadione (MEN) in culture by fluorescent immunohistochemistry (Sobinoff, A.P., et al. (2010). Toxicol. Sci. 118(2):653-666).
Immunocytochemistry Analysis: A representative lot detected significantly increased 8-oxoguanine (8-oxoG) immunoreactivity in RINm5F rat insulinoma cells upon IL-1beta or ROS-inducer menadione treatment (Mehmeti, I., et al. (2011). Biochim. Biophys. Acta. 1813(10):1827-1835).
Immunocytochemistry Analysis: A representative lot detected significantly increased nuclear 8-oxoguanine (8-oxoG) immunoreactivity in AC16 human cardiomyocytes upon infection by the hemoflagellate protozoan parasite Trypanosoma cruzi (Ba, X., et al. (2010). J. Biol. Chem. 285(15):11596-11606).
Immunocytochemistry Analysis: Representative lots detected nutrient-deprivation-induced enhancement of nuclear 8-oxoguanine (8-oxoG) immunoreactivity in HeLa and COS-7 cells by fluorescent immunocytochemistry (Conlon, K.A., et al. (2003). DNA Repair (Amst). 2(12):1337-1352; Conlon, K.A., et al. (2000). J. Histotechnol. 23(1):37-44).
ELISA Analysis: Specificity of a representative lot against 8-oxoguanine was confirmed by competitive ELISA. Competition by dGMP, dAMP, dCMP or TMP was only observed when the concentrations were increased to the millimolar range.
Immunohistochemistry Analysis: A representative lot detected increased nuclear 8-oxoguanine (8-oxoG) immunoreactivity in paraffin-embedded coronal hippocampus sections from mice induced to express mutated mitochondrial isoform Uracil–DNA glycosylase by fluorescent immunohistochemistry (Lauritzen, K.H., et al. (2011). DNA Repair (Amst). 10(6):639-653).
Immunohistochemistry Analysis: A representative lot detected significantly increased nuclear 8-oxoguanine (8-oxoG) immunoreactivity in ovaries excised from neonatal mice upon exposure to ovotoxic agent 4-vinylcyclohexene diepoxide (VCD), methoxychlor (MXC), or menadione (MEN) in culture by fluorescent immunohistochemistry (Sobinoff, A.P., et al. (2010). Toxicol. Sci. 118(2):653-666).
Immunocytochemistry Analysis: A representative lot detected significantly increased 8-oxoguanine (8-oxoG) immunoreactivity in RINm5F rat insulinoma cells upon IL-1beta or ROS-inducer menadione treatment (Mehmeti, I., et al. (2011). Biochim. Biophys. Acta. 1813(10):1827-1835).
Immunocytochemistry Analysis: A representative lot detected significantly increased nuclear 8-oxoguanine (8-oxoG) immunoreactivity in AC16 human cardiomyocytes upon infection by the hemoflagellate protozoan parasite Trypanosoma cruzi (Ba, X., et al. (2010). J. Biol. Chem. 285(15):11596-11606).
Immunocytochemistry Analysis: Representative lots detected nutrient-deprivation-induced enhancement of nuclear 8-oxoguanine (8-oxoG) immunoreactivity in HeLa and COS-7 cells by fluorescent immunocytochemistry (Conlon, K.A., et al. (2003). DNA Repair (Amst). 2(12):1337-1352; Conlon, K.A., et al. (2000). J. Histotechnol. 23(1):37-44).
ELISA Analysis: Specificity of a representative lot against 8-oxoguanine was confirmed by competitive ELISA. Competition by dGMP, dAMP, dCMP or TMP was only observed when the concentrations were increased to the millimolar range.
This Anti-8-Oxoguanine, clone 483.15 Antibody (Ascites Free) is validated for use in Immunocytochemistry, Immunohistochemistry and ELISA for the detection of 8-Oxoguanine.
Jakość
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected 8-Oxoguanine in HeLa cells.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected 8-Oxoguanine in HeLa cells.
Powiązanie
Replaces: MAB3560
Postać fizyczna
Format: Purified
Purified mouse monoclonal IgMκ antibody in PBS without preservatives (azide free).
Inne uwagi
Concentration: Please refer to lot specific datasheet.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Immunofluorescent localization of the murine 8-oxoguanine DNA glycosylase (mOGG1) in cells growing under normal and nutrient deprivation conditions.
Conlon, KA; Zharkov, DO; Berrios, M
DNA Repair null
Ilir Mehmeti et al.
Biochimica et biophysica acta, 1813(10), 1827-1835 (2011-07-26)
Pro-inflammatory cytokine-mediated beta cell apoptosis is activated through multiple signaling pathways involving mitochondria and endoplasmic reticulum. Activation of organelle-specific caspases has been implicated in the progression and execution of cell death. This study was therefore performed to elucidate the effects
M García-Vaquero et al.
Animal : an international journal of animal bioscience, 6(9), 1435-1443 (2012-10-04)
The aim of this paper is to evaluate at a histopathological level the effect of the most commonly used copper (Cu) supplementation (15 mg/kg dry matter (DM)) in the liver of intensively reared beef cattle. This was done by a
Knut H Lauritzen et al.
DNA repair, 10(6), 639-653 (2011-05-10)
Mitochondria are highly dynamic organelles that can be actively transported within the cell to satisfy local requirements. They are vital for providing cellular energy, but are also an important endogenous source of reactive oxygen species. The distribution of mitochondria is
Alexander P Sobinoff et al.
Toxicological sciences : an official journal of the Society of Toxicology, 118(2), 653-666 (2010-09-11)
Mammalian females are born with a finite number of nonrenewing primordial follicles, the majority of which remain in a quiescent state for many years. Because of their nonrenewing nature, these "resting" oocytes are particularly vulnerable to xenobiotic insult, resulting in
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