MAB3540
Anti-Lamin A Antibody, CT, a.a. 598-611, clone 133A2
clone 133A2, Chemicon®, from mouse
Synonim(y):
70 kDa Lamin
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
culture supernatant
rodzaj przeciwciała
primary antibodies
klon
133A2, monoclonal
reaktywność gatunkowa
rat, mouse, canine, bovine, human
producent / nazwa handlowa
Chemicon®
metody
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
izotyp
IgG3
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... LMNA(4000)
Opis ogólny
Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.
Specyficzność
Monoclonal 133A2 reacts against human lamin A but not lamin C. The epitope lies with the 98 amino acids of the C terminus that is unique to lamin A. Specifically deletion analysis has shown that amino acids 598-611 were essential for reactivity {Hozak, P et.al. (1995), J. Cell Sci 635-644}.
Immunogen
Epitope: C-terminus, a.a. 598-611
Synthetic peptide from the C-terminus of human lamin A. This sequence is not present in the the lamin C isoform.
Zastosowanie
Immunoblotting: Lamin A detected as a 70kDa protein under reduced conditions.
Immunocytochemistry on fixed cells (methanol/acetone fixation)
Immunohistochemistry on frozen tissue sections.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry on fixed cells (methanol/acetone fixation)
Immunohistochemistry on frozen tissue sections.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Lamin A Antibody, C-terminus, a.a. 598-611, clone 133A2 is validated for use in FC, WB, IC, IH for the detection of Lamin A.
Postać fizyczna
Format: Purified
Liquid in buffer containing 0.1% sodium azide.
Przechowywanie i stabilność
Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Raju Padiya et al.
PloS one, 9(5), e94228-e94228 (2014-05-07)
Cardiovascular complication due to diabetes has remained a major cause of death. There is an urgent need to intervene the cardiac complications in diabetes by nutritional or pharmacological agents. Thus the present study was designed to find out the effectiveness
Catherine Coffinier et al.
The Journal of biological chemistry, 285(27), 20818-20826 (2010-05-05)
Lamin A, a key component of the nuclear lamina, is generated from prelamin A by four post-translational processing steps: farnesylation, endoproteolytic release of the last three amino acids of the protein, methylation of the C-terminal farnesylcysteine, and finally, endoproteolytic release
J L Broers et al.
Histochemistry and cell biology, 107(6), 505-517 (1997-06-01)
A selection of normal human tissues was investigated for the presence of lamins B1, B2, and A-type lamins, using a panel of antibodies specific for the individual lamin subtypes. By use of immunoprecipitation and two-dimensional immunoblotting techniques we demonstrated that
P Hozák et al.
Journal of cell science, 108 ( Pt 2), 635-644 (1995-02-01)
The nuclear lamina forms a protein mesh that underlies the nuclear membrane. In most mammalian cells it contains the intermediate filament proteins, lamins A, B and C. As their name indicates, lamins are generally thought to be confined to the
Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells.
Neri, L M, et al.
Journal of Cellular Physiology, 178, 284-295 (1999)
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