MAB3446
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9
ascites fluid, clone 2HP-2G9, Chemicon®
Synonim(y):
HP1a, Antigen p25, Chromobox Protein Homolog 5
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
2HP-2G9, monoclonal
reaktywność gatunkowa
mouse, human
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... CBX5(23468)
Opis ogólny
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Specyficzność
Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.
Immunogen
Recombinant mouse HP1alpha.
Zastosowanie
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5 & has been validated in ELISA, IP & WB.
ELISA: 1:500-1:5,000
Western blot: 1:500-1:5,000
Immunoprecipitation: 1:500-1:5,000
Immunohistochemistry/Immunocytochemistry: Not Recommended
Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.
Optimal working dilutions must be determined by the end user.
Western blot: 1:500-1:5,000
Immunoprecipitation: 1:500-1:5,000
Immunohistochemistry/Immunocytochemistry: Not Recommended
Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.
Optimal working dilutions must be determined by the end user.
Opis wartości docelowych
25 kDa
Postać fizyczna
Liquid ascites fluid containing no preservative.
Komentarz do analizy
Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Up-regulation of Rac GTPase activating protein 1 is significantly associated with the early recurrence of human hepatocellular carcinoma.
Wang SM, Ooi LL, Hui KM
Clinical cancer research : an official journal of the American Association for Cancer Research null
Akinori Takahashi et al.
Experimental cell research, 315(7), 1117-1141 (2009-02-28)
Src-family kinases (SFKs), which participate in various signaling events, are found at not only the plasma membrane but also several subcellular compartments, including the nucleus. Nuclear structural changes are frequently observed during transcription, cell differentiation, senescence, tumorigenesis, and cell cycle.
Aurora-B/AIM-1 regulates the dynamic behavior of HP1alpha at the G2-M transition.
Terada, Y
Molecular Biology of the Cell null
A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1.
Obuse, C; Iwasaki, O; Kiyomitsu, T; Goshima, G; Toyoda, Y; Yanagida, M
Nature Cell Biology null
A role for antibodies to human leukocyte antigens, collagen-V, and K-?1-Tubulin in antibody-mediated rejection and cardiac allograft vasculopathy.
Nath DS, Tiriveedhi V, Basha HI, Phelan D, Moazami N, Ewald GA, Mohanakumar T.
Transplantation null
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej