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MAB2510

Sigma-Aldrich

Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2

clone 264-449-2, Chemicon®, from mouse

Synonim(y):

GFP

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

264-449-2, monoclonal

reaktywność gatunkowa (przewidywana na podstawie homologii)

all

opakowanie

antibody small pack of 25 μg

producent / nazwa handlowa

Chemicon®

metody

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG1

numer dostępu UniProt

Warunki transportu

ambient

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Specyficzność

Directed against a bacterially-expressed fusion protein. Also reacts with red-shifted GFP-variant. Does not react with enhanced GFP.

Immunogen

Epitope: original GFP only
highly-purified native GFP

Zastosowanie

Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2 is an antibody against Green Fluorescent Protein for use in IP, WB & IC.
Immunocytochemistry

Immunoblotting: 1:1000

Immunoprecipitation

Optimal working dilutions and protocols must be determined by the end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Jakość

Tested

Postać fizyczna

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.

Przechowywanie i stabilność

Store at 2-8°C for up to 12 months.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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