MAB2226

Anti-Piccolo Antibody, clone 4G3.3

clone 4G3.3, from mouse

• Synonim(y): presynaptic cytomatrix protein, Aczonin
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 4G3.3, monoclonal
• reaktywność gatunkowa: mouse
• reaktywność gatunkowa (przewidywana na podstawie homologii): human (based on 82% homology), rat (92% homology)
• metody: immunohistochemistry: suitable (paraffin); western blot: suitable
• izotyp: IgMκ
• numer dostępu NCBI: NP_001104266
• numer dostępu UniProt: Q9Y6V0
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... PCLO(27445) ; mouse ... Pclo(26875)

Opis

Opis ogólny

Piccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE].

Specyficzność

This antibody recognizes piccolo.

Immunogen

GST-tagged recombinant protein

Zastosowanie

Immunohistochemistry (Paraffin):
Representative images from a previous lot.
Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

This Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo.

Jakość

Routinely evaluated by western blot on rat brain lysate.

Opis wartości docelowych

547 kDa

Postać fizyczna

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS and 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Rat brain lysate, mouse cerebellum

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable