MAB1680
Anti-Filamin A Antibody, clone TI10
ascites fluid, clone TI10, Chemicon®
Synonim(y):
Alpha-Filamin, Filamin I, Endothelial Actin-binding Protein, ABP-280, Nonmuscle Filamin
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
TI10, monoclonal
reaktywność gatunkowa
human, bovine
spodziewany brak reakcji z
rat, mouse
producent / nazwa handlowa
Chemicon®
metody
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
mouse ... Flna(192176)
Opis ogólny
Filamin is a structural protein that forms flexible cross-links between two actin filaments. Filamin is a homodimer of polypeptide chains each joined to the other at one end with an actin binding site ath the other. It is present in smooth muscle, fibroblasts, platelets and lymphocytes.
Specyficzność
Human filamin (actin-bidning protein). Recognizes unprocessed (270-280 kDa) and the C-terminal 90 kDa calpain cleavage fragment of filamin (Aakhus, 1992).
Immunogen
Human platelet protein
Zastosowanie
Detect Filamin A using this Anti-Filamin A Antibody, clone TI10 validated for use in IP, WB, IH, IH(P).
Immunoblotting: 1:250 to 1:1000
Immunoprecipitation:Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for every 300μL of cell lysate (3-5mg/ml total protein is suggested). Incubation is 4 hours RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are recommended for full length filamin or the 80kDa fragement visualization.
Immunofluorescence: 1:50 to 1:200 using standard ABC technique. Suitable for staining of paraffin embedded sections (lower dilutions). High temperature citrate buffer antigen retrieval technique recommended.
Optimal working dilutions must be determined by end user.
Immunoprecipitation:Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for every 300μL of cell lysate (3-5mg/ml total protein is suggested). Incubation is 4 hours RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are recommended for full length filamin or the 80kDa fragement visualization.
Immunofluorescence: 1:50 to 1:200 using standard ABC technique. Suitable for staining of paraffin embedded sections (lower dilutions). High temperature citrate buffer antigen retrieval technique recommended.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Powiązanie
Replaces: CBL229
Postać fizyczna
Ascites. Liquid
Przechowywanie i stabilność
Maintain frozen at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze/thaw cycles.
Komentarz do analizy
Control
Positive control tisse: skin.
Positive control tisse: skin.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
A 90 kDa fragment of filamin A promotes Casodex-induced growth inhibition in Casodex-resistant androgen receptor positive C4-2 prostate cancer cells.
Wang, Y; Kreisberg, JI; Bedolla, RG; Mikhailova, M; deVere White, RW; Ghosh, PM
Oncogene null
Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis.
Jian-Feng Lin,Jun Xu,Hong-Yu Tian,Xia Gao,Qing-Xi Chen,Qi Gu,Gen-Jun Xu,Jian-da Song,Fu-Kun Zhao
International Journal of Cancer. Journal International Du Cancer null
Kun Wang et al.
Molecular medicine reports, 20(4), 3671-3678 (2019-09-06)
The metastasis and recurrence rate, and the overall prognosis of colorectal cancer (CRC) remain unsatisfactory. Filamin A (FLNa), as an actin‑binding protein, can interact with various signaling molecules and membrane receptors to affect cell signal transduction and function. However, whether
Structure of three tandem filamin domains reveals auto-inhibition of ligand binding.
Yatish Lad,Tiila Kiema,Pengju Jiang,Olli T Pentikainen,Charlotte H Coles,Iain D Campbell et al.
The Embo Journal null
Enhancing the effectiveness of androgen deprivation in prostate cancer by inducing Filamin A nuclear localization.
Mooso, Benjamin A, et al.
Endocrine-Related Cancer, 19, 759-777 (2012)
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej