MAB16200
Anti-MGMT Antibody, clone MT3.1
clone MT3.1, Chemicon®, from mouse
Synonim(y):
O-6-methylguanine-DNA Methyltransferase, Methylated-DNA--Protein-cysteine Methyltransferase
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
MT3.1, monoclonal
reaktywność gatunkowa
human
producent / nazwa handlowa
Chemicon®
metody
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... MGMT(4255)
Opis ogólny
O6-Methylguanine-DNA Methyltransferase (MGMT) is a ubiquitous DNA repair protein that removes O6-alkyl-guanine, primarily O6 methylguanine lesions from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation. The mechanism of MGMT action is based on the transfer of the alkyl group from the DNA to a unique acceptor cysteine residue in the protein, forming a stable thioether linkage. MGMT is frequently classified as a suicide protein. The repair capacity for O6- methylguanine is dependent on the number of MGMT molecules in the cell. There is a correlation between the occurrence of cancer in various tissues and the lack of the MGMT enzyme. High levels of MGMT result in reduced tumor events and resistance of tumors to alkylating agents.
Specyficzność
MGMT is a 22 kDa DNA repair protein that is present in all normal tissues; however, in a subset of human tumours MGMT is completely absent. Thus, antibody MAB16200 may be helpful in utilizing MGMT as a marker of malignant or pre-malignant cells. Because MGMT repairs the potentially cytotoxic antitumour lesions induced in DNA by certain cancer chemotherapeutic alkylating agents (e.g. BCNU, CCNU, DTIC, procarbazinc, temozolomide), tumours lacking or having low levels of MGMT will predictably be responsive to drug therapy. Conversely, tumours with high MGMT levels will likely be drug resistant. The MGMT antibody may also be used to gauge the effectiveness of MGMT modulators, such as O-6-benzylguanine, which lead to rapid degradation of the inactivated protein.
Immunogen
Made against recombinant human MGMT (O-6-methylguanine-DNA methyltransferase) expressed in E. coli.
Zastosowanie
Flow Cytometry:
An independent laboratory used a previous lot on flow cytometry (Gerson, 1996).
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Immunohistochemistry:
A previous lot of this antibody was used on paraffin embedded sections.
Optimal working dilutions must be determined by end user.
An independent laboratory used a previous lot on flow cytometry (Gerson, 1996).
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Immunohistochemistry:
A previous lot of this antibody was used on paraffin embedded sections.
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-MGMT Antibody, clone MT3.1 (mouse monoclonal antibody) validated in FC, ICC,IHC(P), IP, WB to detect MGMT also known as O-6-methylguanine-DNA Methyltransferase, Methylated-DNA--Protein-cysteine Methyltransferase.
Useful for detection of MGMT in human tissues, tumours, cells, or xenograft lines. Gives weak cross-reactivity with MGMT from mouse, hamster and rat cells.
Jakość
Evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected MGMT on 10 µg of Jurkat lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected MGMT on 10 µg of Jurkat lysates.
Opis wartości docelowych
22-25 kDa
Postać fizyczna
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB, pH 7.6, 0.25 M NaCl containing 0.1% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8ºC from date of receipt.
Komentarz do analizy
Control
Positive Control: Tonsil tissue, HeLa cells, CEM-CCRF cells.
Negative Control: TK6 cells.
Positive Control: Tonsil tissue, HeLa cells, CEM-CCRF cells.
Negative Control: TK6 cells.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Wild-type p53 suppresses transcription of the human O6-methylguanine-DNA methyltransferase gene.
Harris, L C, et al.
Cancer Research, 56, 2029-2032 (1996)
Yuichi Sato et al.
Cancer biology & therapy, 8(5), 452-457 (2009-03-24)
Phosphorylation of histone H2AX is a sensitive marker of DNA damage, particularly of DNA double strand breaks. Using multiparameter cytometry we explored effects of etoposide and temozolomide (TMZ) on three glioblastoma cell lines with different p53 status (A172, T98G, YKG-1)
Netta Levin et al.
Cancer, 106(8), 1759-1765 (2006-03-17)
Loss of heterozygosity (LOH) on chromosomes 1p and 19q has been associated with chemosensitivity and improved prognosis in patients with oligodendrogliomas. The DNA repair enzyme O6-methylguanine DNA methyltransferase (MGMT) may induce resistance to DNA-alkylating agents. Recent studies demonstrated that temozolomide
Masaki Yoshioka et al.
Oncotarget, 9(45), 27728-27735 (2018-07-03)
The methylation status of the O6-methylguanine-DNA methyltransferase (MGMT) gene is a strong predictor for the efficacy of temozolomide chemotherapy and survival periods. However, the correlation between the extent of methylation and the difference in survival times has not been fully
Longitudinal assessment of genetic and epigenetic markers in oligodendrogliomas.
Lavon, I; Zrihan, D; Zelikovitch, B; Fellig, Y; Fuchs, D; Soffer, D; Siegal, T
Clinical cancer research : an official journal of the American Association for Cancer Research null
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