ABS989

Anti-E6AP Antibody

serum, from rabbit

• Synonim(y): Ubiquitin-protein ligase E3A, E6AP ubiquitin-protein ligase, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: serum
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• reaktywność gatunkowa: human, mouse, rat
• metody: immunoprecipitation (IP): suitable; western blot: suitable
• izotyp: IgG
• numer dostępu NCBI: NP_000453
• numer dostępu UniProt: Q05086
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... UBE3A(7337)

Opis

Opis ogólny

E6AP/UB3A is an interesting E3 ubiquitin protein ligase with newly discovered alternative functions as well. It is the founding member of the HECT (Homologous to E6AP C terminus) family of ubiquitin ligases and recent research is shedding new light on its influence in health and disease. Ubiquitin substrates for E6AP/UB3A ligase include RAD23A/B, MCM7 (items involved in DNA replication), Annexin A1, PML tumor suppressor and the cell cycle regulator CDKN1B. E6AP also catalyzes the high risk human papilloma virus E6 mediated ubiquitination of p53, which contributes to the neoplastic progression of cells infected by these viruses. E6AP has also been identified as a steroid hormone receptor transcriptional coactivator, and E6AP has a role in numerous diseases including cancers and neurological syndromes. Additionally, E6AP/UB3A is developmentally important for adipogenesis, granulocyte maturation and synaptic processes, where it acts as a down-regulator. IE6AP/UBE3A protein is active as a trimer of E6AP subunits and widely expressed.

Immunogen

a small portion of the full length protein expressed as a recombinant

Zastosowanie

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-E6AP Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of E6AP.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected E6AP in 10 µg of A431, C2C12, L6, mouse brain, and PC3 cell lysate.
Western Blotting Analysis: A representative lot detected E6AP in HeLa cell lysate (Talis, A. L., et al. (1998). JBC. 273:6439-6445).
Immunoprecipitation Analysis: A representative lot immunoprecipitated E6AP in HeLa cell lysate (Wynn, H. K., et al. (2000). Journal of Virology. 74(14):6408-6417).

Jakość

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected E6AP in 10 µg of A431 cell lysate.

Opis wartości docelowych

~100 kDa observed. Uncharacterized band(s) may appear in some lysates.

Postać fizyczna

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1