ABS31-I

Anti-2A Peptide

• Synonim(y): 2A Peptide Antibody
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 200
• białko sprzężone: unconjugated
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• masa cząsteczkowa: calculated mol wt 258.93 kDa; observed mol wt ~21, 26, and 31 kDa
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: all
• opakowanie: antibody small pack of 100 μg
• metody: affinity binding assay: suitable; western blot: suitable
• izotyp: IgG
• sekwencja epitopowa: Internal
• numer dostępu UniProt: P03305
• Warunki transportu: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: Unknown ... D1Y31_gp1(37627025)

Opis

Opis ogólny

Genome polyprotein (UniProt: P03305) is encoded by the D1Y31_gp1 gene (Gene ID: 37627025) in Foot-and-mouth disease virus. Following its synthesis, it is cleaved into 15 different chains. P2A chain resides in the amino acids 936-953 in the internal region. 2A peptides and 2A-like peptide sequences (known as cis-acting hydrolase elements or CHYSEL) are a superior alternative to internal ribosomal entry sites (IRES) for coordinating the expression of multiple gene products from a single recombinant construct. The 2A sequences are relatively short peptides of about 16-20 amino acids (depending on the virus of origin). They contain a highly conserved sequence, GDVEXNPGP, at the C-terminus, which is shared by different 2As and is essential for the creation of steric hindrance and ribosome skipping. These peptides allow multiple proteins to be encoded as polyproteins, which then dissociate into component proteins upon translation. The 2A sequence impairs normal peptide bond formation via a mechanism called ribosomal skipping, which results in effective, non-enzymatic generation of distinct peptide products from a single multi-cistronic construct. These peptides are used by several families of viruses, the best-known amongst these are foot-and-mouth disease virus 2A (F2A) and Thosea asigna virus 2A (T2A). This rabbit polyclonal antibody reacts with protein in lysate from HEK293T cells transfected with various 2A-tagged proteins. (Ref.: Liu, Z., et al. (2017). Scientific Reports.7; Article number: 2193).

Specyficzność

This rabbit polyclonal antibody detects 2A peptide tagged proteins.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids (936-953) 2A peptide of Foot-and-mouth disease virus.

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cells transfected with CD3/g/d/e chains.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected 2A in lysate from HEK293T cells transfected with CD3/g/d/e chains.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound 2A peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-2A Peptide, Cat. No. ABS31-I, is a rabbit polyclonal antibody that detects 2A peptide tagged proteins and is tested for use in Affinity Binding Assay and Western Blotting.

Postać fizyczna

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable