ABE413
Anti-NF-E2-related factor 2 Antibody
from rabbit, purified by affinity chromatography
Synonim(y):
Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor erythroid derived 2-like 2
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
human
reaktywność gatunkowa (przewidywana na podstawie homologii)
mouse (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), rat (based on 100% sequence homology)
opakowanie
antibody small pack of 25 μg
metody
immunocytochemistry: suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
ambient
temp. przechowywania
2-8°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... NFE2L2(4780)
Powiązane kategorie
Opis ogólny
NF-E2-related factor 2 (Nuclear factor erythroid 2-related factor 2) is a transcription activator that binds to antioxidant response elements (ARE) in the promoter regions of target genes. NF-E2-related factor 2 mediates the coordinated up-regulation of genes in response to oxidative stress. NF-E2-related factor 2 may also be involved in the transcriptional activation of genes of the beta-globin cluster. NF-E2-related factor 2 is widely expressed, with highest expression in adult muscle, kidney, lung, liver and fetal muscle.
Specyficzność
This antibody recognizes the C-terminus of NF-E2 related factor 2
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human NF-E2 related factor 2.
Zastosowanie
Anti-NF-E2-related factor 2 Antibody is a highly specific rabbit polyclonal antibody, that targets NF-E2-related factor 2 & has been tested in western blotting & ICC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Western Blotting Analysis: 1 µg/mL from a representative lot detected NF-E2 related factor 2 in 10 µg of Jurkat cell lysate.
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected NF-E2 related factor 2 in cells untreated and treated with hydrogen peroxide (Note: Cytoplasmic staining is increased under oxidative stress.).
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected NF-E2 related factor 2 in cells untreated and treated with hydrogen peroxide (Note: Cytoplasmic staining is increased under oxidative stress.).
Jakość
Evaluated by Western Blotting in Ramos cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected NF-E2 related factor 2 in 10 µg of Ramos cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected NF-E2 related factor 2 in 10 µg of Ramos cell lysate.
Opis wartości docelowych
~75 kDa observed. Two isoforms at 68 kDa (isoform 1) and 66 kDa (isoform 2) are known to exist. This protein has been observed at ~75 kDa due to the abundance of acidic residues found in Nrf2, which causes it to migrate anomalously in SDS-PAGE (Abu-Bakar, A., et al. (2007) Drug Metab Dispos. 35(5):787-794.). A ubiquitinated form of NF-E2-related factor 2 may be observed at ~110 kDa (Li, J., et al. (2005). Toxicol Sci. 83(2):313-328.).
Postać fizyczna
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Komentarz do analizy
Control
Ramos cell lysate
Ramos cell lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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