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Key Documents

ABE2622

Sigma-Aldrich

Anti-SYCP2

serum, from rabbit

Synonim(y):

Synaptonemal complex protein 2, SCP-2, Synaptonemal complex lateral element protein

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

forma przeciwciała

serum

rodzaj przeciwciała

primary antibodies

klon

polyclonal

reaktywność gatunkowa

mouse, human

metody

immunofluorescence: suitable
western blot: suitable

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

ambient

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... SYCP2(10388)
mouse ... Sycp2(320558)

Opis ogólny

Synaptonemal complex protein 2 (UniProt: Q9CUU3; also known as SCP-2, SYCP2, Synaptonemal complex lateral element protein) is encoded by the Sycp2 (also known as Scp2) gene (Gene ID: 320558) in murine species.SYCP2 is present in testis and spermatocyte and is a major component of the axial/lateral elements of synaptonemal complexes (SCS) during meiotic prophase and plays a role in the assembly of synaptonemal complexes. It is required for normal meiotic chromosome synapsis during oocyte and spermatocyte development and for normal male and female fertility. SYCP2 is considered to be a primary determinant of axial/lateral elements and can heterodimerize with SYCP3 and it is is required for the incorporation of SYCP3 into the synaptonemal complex. An evolutionarily conserved coiled coil domain in SYCP2 is required for binding to SYCP3. Defects in SYCP2 leads to sterility in male mice due to defective meiotic chromosome synapsis during spermatocyte development. Also, SYCP2 deficient mice show much reduced testes weight.

Specyficzność

This polyclonal antibody detects Synaptonemal complex protein 2 in mouse testis and recognizes an epitope within amino acids 1255-1500 in the C-terminal region.

Immunogen

Epitope: C-terminus
His-tagged recombinant fragment corresponding to 246 amino acids from the C-terminal region of murine Synaptonemal complex protein 2.

Zastosowanie

Anti-SYCP2 , Cat. No. ABE2622, is a highly specific rabbit polyclonal antibody that targets Synaptonemal complex protein 2 and has been tested in Immunofluorescence and Western Blotting.
Immunofluorescence Analysis: A representative lot detected SYCP2 in spread nuclei of mouse spermatocytes. (Courtesy of Dr. P. Jeremy Wang at University of Pennsylvania).

Immunofluorescence Analysis: A representative lot detected SYCP2 in spread nuclei of oocytes and spermocytes (Yang, F., et. al. (2015). EMBO Mol Med. 7(9):1198-210).

Western Blotting Analysis: A representative lot detected SYCP2 in adult mouse testis tissue lysate (Yang, F., et. al. (2006). J Cell Biol. 173(4):497-507).
Research Category
Epigenetics & Nuclear Function

Jakość

Evaluated by Western Blotting in mouse testis tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SYCP2 in 50 µg of mouse testis tissue lysate.

Opis wartości docelowych

~200 kDa observed; 172.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Rabbit polyclonal anti-serum with 0.05% sodium azide.
Unpurified

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1


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Odwiedź Bibliotekę dokumentów

Samantha L P Schilit et al.
American journal of human genetics, 106(1), 41-57 (2019-12-24)
Unexplained infertility affects 2%-3% of reproductive-aged couples. One approach to identifying genes involved in infertility is to study subjects with this clinical phenotype and a de novo balanced chromosomal aberration (BCA). While BCAs may reduce fertility by production of unbalanced

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