ABE155

Anti-phospho Histone H3.1/H3.2 Antibody (Ser87)

from rabbit, purified by affinity chromatography

• Synonim(y): Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: bovine, mouse, human
• metody: dot blot: suitable; western blot: suitable
• numer dostępu NCBI: NP_003520
• numer dostępu UniProt: P68431
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pSer87)
• informacje o genach: human ... SNCA(6622)

Opis

Opis ogólny

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3.1, H3.2 and H3.3 are all closely related Histone H3 variants, that despite differing by only a few amino acids have been shown to have different functional roles.

Specyficzność

This antibody recognizes Histone H3.1/H3.2 phosphorylated at Ser87. This antibody demonstrates specificity for phospho Histone H3.1/H3.2 (Ser87), but does not cross react with phospho Histone H3.3, or any other modified and non-modified histones.

Immunogen

Epitope: Phosphorylated at Ser87

KLH-conjugated linear peptide corresponding to human Histone H3.1/H3.2 phosphorylated at Ser87.

Zastosowanie

Detect Histone H3 using this rabbit polyclonal antibody, Anti-phospho Histone H3.1/H3.2 Antibody (Ser87) validated for use in western blotting & Dot Blot.

Dot Blot (Specificity) Analysis: 1µg/mL of this antibody detected phospho Histone H3.1/H3.2 (Ser87) in a panel of unmodified and modified Histones.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Jakość

Evaluated by Western Blotting in Colcemid treated HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho Histone H3.1/H3.2 (Ser87) in 10 µg of Colcemid treated HeLa acid extract

Opis wartości docelowych

~17 kDa observed

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
HeLa acid extract

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable