ABC927

Anti-LDHB Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): L-lactate dehydrogenase B chain (EC:1.1.1.27), LDH-B, LDH heart subunit, LDH-H, Renal carcinoma antigen NY-REN-46, LDHB
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• reaktywność gatunkowa (przewidywana na podstawie homologii): rat (based on 100% sequence homology), canine (based on 100% sequence homology), mouse (based on 100% sequence homology), rabbit (based on 100% sequence homology), bovine (based on 100% sequence homology), pig (based on 100% sequence homology), horse (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• metody: immunohistochemistry: suitable (paraffin); western blot: suitable
• numer dostępu NCBI: NP_002291
• numer dostępu UniProt: P07195
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... LDHB(3945)

Opis

Opis ogólny

L-lactate dehydrogenase B chain (EC 1.1.1.27; UniProt P07195; also known as Epididymis secretory protein Li 281, Lactate dehydrogenase H chain, LDH-B, LDH-H, LDH heart subunit, Renal carcinoma antigen NY-REN-46) is encoded by the LDHB (also known as LDHBD, TRG-5, HEL-S-281) gene (Gene ID 3945) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential targets for cancer treatment. Lactate dehydrogenase (LDH) is a tetrameric enzyme composed of two different subunits, LDHA and LDHB, which can assemble into five different combinations (LDH1 is comprised of four LDHB subunits; LDH2 contains three LDHB and one LDHA subunit; LDH3 contains two LDHB and two LDHA subunits; LDH4 contains one LDHB and three LDHA subunits; LDH5 contains four LDHA subunits). LDHB is ubiquitously expressed and the predominant isoform in heart muscle, while LDHA is the predominant isoform in skeletal muscle and other highly glycolytic tissues. LDH catalyzes the reversible conversion of pyruvate into lactate. An upregulation of LDH therefore reduces the oxygen dependency and ensures an efficient anaerobic/glycolytic metabolism in tumor cells. Serum LDHB levels, for example, are reported to elevate in lung cancer patients and progressively increase with the clinical stage of non-small cell lung cancer (NSCLC).

Immunogen

KLH-conjugated linear peptide corresponding to human LDHB sequence from the C-terminal half.

Zastosowanie

Detect LDHb using this rabbit polyclonal Anti-LDHB Antibody, Cat. No. ABC927, validated for use in Western Blotting and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected LDHB in normal human prostate tissue, as well as human cancer cells in Non-Hodgkin lymphoma, kidney, colon, and lung tumor tissues.

Jakość

Evaluated by Western Blotting in human PANC-1 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected LDHB in 10 µg of human PANC-1 cell lysate.

Opis wartości docelowych

~37 kDa observed

Inne uwagi

Concentration: Please refer to lot specific datasheet.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable