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Key Documents

AB1792P

Sigma-Aldrich

Anti-Dopamine D2 Receptor Antibody, cytoplasmic domain, long form

Chemicon®, from rabbit

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

forma przeciwciała

affinity purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

polyclonal

oczyszczone przez

affinity chromatography

reaktywność gatunkowa

canine, rat

producent / nazwa handlowa

Chemicon®

metody

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

dry ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

Specyficzność

Specific for Dopamine Receptor D2 long form. Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L) forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29 amino acid in the intracellular, cytoplasmic loop 3 (Dal Toso et al. 1989; Grandy et al. 1989; Monsma et al. 1990; Giros et al. 1989). D2L receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (Boundy et al. 1993) The 27 aa rat D2RL peptide used for immunization was found only in the D2 long form and there is no significant homology with other dopamine receptors (D1, D2 short, and D3-D5).

SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 96% with human/bovine (26/27 aa), 62% with Xenopus (17/27 aa) D2L receptors. Antibodies generated to the rat sequence have been shown to recognize the canine D2R (Boundy et al. 1993). Antibody cross-reactivity with various species is not known.

Immunogen

A 27 amino acid peptide sequence from the rat D2 long form within the cytoplasmic loop #3.
Epitope: cytoplasmic domain, long form

Zastosowanie

Detect Dopamine D2 Receptor using this Anti-Dopamine D2 Receptor Antibody, cytoplasmic domain, long form validated for use in ELISA, IH, IP & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot: 1-10 μg/mL (Chemiluminescence technique) The predicted size of D2L form is approximately 50 kDa. However, additional bands at approximately 60 and 100 kDa, and others are typically seen in various tissues (Boundy, et al. 1993; Sakata, et al. 1992; Farooqui et al. 1991, 1992; Brock et al. 1992; Farooqui & Prasad 1992). Approximately 100-400 μg of membrane protein/lane should be loaded to visualize various bands.

Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections.

Immunoprecipitation: 1-10 μg using 100-400 μg of protein (Boundy, et al. 1993).

ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (AG211)/well)

Optimal working dilutions must be determined by the end user.

Postać fizyczna

Affinity purified immunoglobulin. Liquid at 1.0 mg/mL in PBS, pH 7.4 with 0.1% BSA and 0.02% sodium merthiolate.

Przechowywanie i stabilność

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Abu Hasanat Md Zulfiker et al.
Journal of cellular biochemistry, 118(5), 979-993 (2016-06-14)
Recent evidences show that activation of serotonin 2A receptors (5-HT2A R) by agonists is significant in improving therapeutic activity of disease conditions, such as obsessive-compulsive disorder (OCD). Though the exact molecular mechanism is still not well understood, it is thought
Single and binge methamphetamine administrations have different effects on the levels of dopamine D2 autoreceptor and dopamine transporter in rat striatum.
Chauhan, H; Killinger, BA; Miller, CV; Moszczynska, A
International Journal of Molecular Sciences null
An antibody to dopamine D2 receptor inhibits dopamine antagonist and agonist binding to dopamine D2 receptor cDNA transfected mouse fibroblast cells.
Farooqui, S M and Prasad, C
Life Sciences, 51, 1509-1516 (1992)
B Giros et al.
Nature, 342(6252), 923-926 (1989-12-21)
Dopamine receptors are classified into D1 and D2 subtypes on the basis of their pharmacological properties and the intracellular responses they mediate. The cerebral D2 dopamine receptor is the target of drugs used to alleviate the main symptoms of schizophrenia.
Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum.
Farooqui, S M, et al.
Journal of Neurochemistry, 57, 1363-1369 (1991)

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