07-441

Anti-dimethyl-Histone H3 (Lys9) Antibody

Upstate^®, from rabbit

• Synonim(y): H3K9me2, Histone H3 (di methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• reaktywność gatunkowa: human, mouse, Schizosaccharomyces pombe, rat, chicken
• producent / nazwa handlowa: Upstate^®
• metody: dot blot: suitable; immunocytochemistry: suitable; inhibition assay: suitable (peptide); western blot: suitable
• izotyp: IgG
• numer dostępu NCBI: NM_003493
• numer dostępu UniProt: Q16695
• Warunki transportu: dry ice
• docelowa modyfikacja potranslacyjna: dimethylation (Lys9)
• informacje o genach: human ... H3C1(8350) ; mouse ... H3C1(360198) ; rat ... H3C1(679994)

Opis

Opis ogólny

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specyficzność

Expected to cross react with mouse, chicken, rat and fission yeast, but not budding yeast. Broad species cross-reactivity expected.

Immunogen

KLH-conjugated, 2X-branched synthetic peptide containing the sequence ..Rme2KSTG.., in which me2K corresponds to dimethyl-lysine at residue 9 of human Histone H3.

Zastosowanie

Dot Blot Analysis:
Specificity confirmed by an independent laboratory.

Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of Histone H3 in western blot analysis of HeLa acid extracts (Figure A, Lane 4).

Immunocytochemistry:
Reported by an independent laboratory.

Use Anti-dimethyl-Histone H3 (Lys9) Antibody (Rabbit Polyclonal Antibody) validated in DB, ICC, PIA, WB to detect dimethyl-Histone H3 (Lys9) also known as H3K9me2, Histone H3 (di methyl K9).

Jakość

Routinely evaluated by Western Blot on HeLa acid extract and recombinant Histone H3 (cat. # 14-411).

Western Blot Analysis:
1:500 dilution of this lot detected dimethyl Histone H3 on 10 μg of HeLa acid extract but not on recombinant Histone H3.

Opis wartości docelowych

17 kDa

Postać fizyczna

Format: Purified

Przechowywanie i stabilność

1 year at -20°C from date of shipment.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Komentarz do analizy

Control
HeLa whole cell lysate, U2OS cell lysate.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1