AB1761-I
Anti-Protein Gene Product 9.5 Antibody
from rabbit, purified by affinity chromatography
Synonim(y):
Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
human, mouse, rat
reaktywność gatunkowa (przewidywana na podstawie homologii)
bovine (based on 100% sequence homology), equine (based on 100% sequence homology), porcine (based on 100% sequence homology)
metody
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... UCHL1(7345)
Opis ogólny
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UniProt: P09936; also known as EC: 3.4.19.12, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1) is encoded by the UCHL1 gene (Gene ID: 7345) in human. UCH-L1 is a member of the peptidase C12 family that can exist as a monomer or homodimer. It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors and is detected in neuronal cell bodies and processes throughout the neocortex. It displays a weak expression in ovary. About 30% of total UCH-L1 is reported to be associated with membranes in brain. Its levels are shown to be down-regulated in brains from Parkinson disease and Alzheimer disease patients. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin and is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimeric form may have ATP-independent ubiquitin ligase activity. Mutations in UCHL1 gene are known to cause Parkinson disease 5, a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Some mutations in this gene are also linked to spastic paraplegia 79, where patients display a form of spastic paraplegia, which is characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Immunogen
KLH-conjugated linear peptide corresponding to human Protein Gene Product 9.5.
Zastosowanie
Detect PGP9.5 using this rabbit polyclonal antibody, Anti-Protein Gene Product 9.5 Antibody validated for use in western blotting, IHC (Paraffin), Immunofluorescence & ICC.
Immunofluorescence Analysis: A 1:200 dilution from a representative lot detected Gene Product 9.5 in human cerebellum.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Gene Product 9.5 in human cerebellum.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Gene Product 9.5 in E18 rat cortical cells..
Dylight® is a registered trademark of Thermo Fisher Scientific.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Gene Product 9.5 in human cerebellum.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Gene Product 9.5 in E18 rat cortical cells..
Dylight® is a registered trademark of Thermo Fisher Scientific.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Jakość
Evaluated by Western Blotting in rat brain, human brain, and mouse brain tissue lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Protein Gene Product 9.5 (UCH-L1) in rat brain, human brain, and mouse brain tissue lysate
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Protein Gene Product 9.5 (UCH-L1) in rat brain, human brain, and mouse brain tissue lysate
Opis wartości docelowych
~24 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Powiązanie
Replaces: AB1761 and AB9724
Postać fizyczna
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH7.4 with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
DyLight is a registered trademark of Pierce Biotechnology, Inc.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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