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KOD Hot Start Master Mix

ready-to-use solution, Ready-to-use 2X mixture, containing KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultrapure deoxynucleotides, and reaction buffer with MgSO4, optimized for convenient high fidelity PCR., suitable for PCR

Synonim(y):

Gorący start PCR, Mieszanka wzorcowa z gorącym rozruchem, Wysokiej jakości master mix

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About This Item

Kod UNSPSC:
41105500
NACRES:
NA.55

Poziom jakości

100

Formularz

ready-to-use solution

zastosowanie

sufficient for 100 reactions
 sufficient for 500 reactions

Właściwości

Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included
hotstart

producent / nazwa handlowa

Novagen®

warunki przechowywania

OK to freeze

metody

PCR: suitable (2X PCR Master Mix for high fidelity)
PCR: suitable

moc wejściowa

purified DNA

Warunki transportu

dry ice

temp. przechowywania

−20°C

Powiązane kategorie

Opis ogólny

KOD Hot Start Master Mix is a ready-to-use 2X mixture optimized for convenient high-fidelity PCR, which utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. The mix contains KOD Hot Start DNA Polymerase, two monoclonal antibodies, ultra-pure deoxynucleotides, and reaction buffer with MgSO4. The monoclonal antibodies inhibit the DNA polymerase and 3′→5′ exonuclease activities at ambient temperatures.

Zastosowanie

KOD Hot Start Master Mix has been used:
  • To yield PCR product for T7 endonuclease I (T7EI) assay.
  • For PCR amplification of the DNA isolated from Chrysosporium multifidum, a fungus with antimicrobial activity.
  • In the PCR amplification of cDNA.
  • In two-step PCR for the amplification of the target sequence followed by its insertion into a vector.

Cechy i korzyści

  • Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
  • Amplifies genomic DNA templates up to 12 kbp
  • Amplifies plasmid and lambda DNA template up to 21 kbp
  • Eliminates mispriming and primer-dimer formation
  • Convenient ambient-temperature setup compatible with automation
  • KOD HotStart® Buffer ensures optimal PCR performance over a wide range of targets.
  • Offers high specificity, fidelity and yield during PCR amplification
  • Optimized for ease of use and data reproducibility
  • Simplifies PCR set-up and saves time
  • High consistency with minimal risk of contamination

*Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).

Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

Komponenty

  • 2 × 1.25 ml or 10 × 1.25 mL KOD Hot Start Master Mix

References:
Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem.66 (10), 2194. Fujii, S., et al. 1999. J. Mol.

Ostrzeżenie

Toxicity: Standard Handling (A)

Definicja jednostki

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA.

Inne uwagi

Simply add KOD HotStart® Master Mix to an equal volume of sample containing DNA template and primers. The final diluted reaction contains 1U KOD HotStart® DNA Polymerase per 50 μl reaction. The smaller available size provides sufficient master mix for 100 (50 μl scale), or 250 (20 μl scale) reactions, while the larger size is adequate for 500 (50 μl scale) or 1250 (20 μl scale) reactions.

Informacje prawne

* Manufactured by Toyobo and distributed by Merck KGaA, Darmstadt, Germany and/or its affiliates (not in Japan).Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Kim A Lagerborg et al.
bioRxiv : the preprint server for biology (2021-03-25)
The rapid global spread and continued evolution of SARS-CoV-2 has highlighted an unprecedented need for viral genomic surveillance and clinical viral sequencing. Amplicon-based sequencing methods provide a sensitive, low-cost and rapid approach but suffer a high potential for contamination, which
Kim A Lagerborg et al.
Nature microbiology, 7(1), 108-119 (2021-12-16)
The global spread and continued evolution of SARS-CoV-2 has driven an unprecedented surge in viral genomic surveillance. Amplicon-based sequencing methods provide a sensitive, low-cost and rapid approach but suffer a high potential for contamination, which can undermine laboratory processes and
Kothai Parthiban et al.
mAbs, 11(5), 884-898 (2019-05-21)
The construction of large libraries in mammalian cells allows the direct screening of millions of molecular variants for binding properties in a cell type relevant for screening or production. We have created mammalian cell libraries of up to 10 million
Yesenia Correa et al.
PloS one, 14(12), e0218837-e0218837 (2019-12-21)
The gut microbiota of insects is composed of a wide range of microorganisms which produce bioactive compounds that protect their host from pathogenic attack. In the present study, we isolate and identify the fungus Chrysosporium multifidum from the gut of
Fantao Kong et al.
Bio-protocol, 8(3), e2718-e2718 (2018-02-05)
Chlamydomonas reinhardtii is frequently used as a model organism to study fundamental processes in photosynthesis, metabolism, and flagellar biology. Versatile tool boxes have been developed for this alga ( Fuhrmann et al., 1999 ; Schroda et al., 2000 ; Schroda
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Produkty

PCR Master Mix

A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.

PCR Master Mix

Mieszanina wzorcowa PCR upraszcza PCR/RT-PCR dzięki składnikom takim jak polimeraza DNA, dNTP, MgCl2 i bufor, dostępnym w handlu lub DIY.

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

Hot Start PCR

Celem Hot Start PCR jest zahamowanie reakcji PCR w celu zmniejszenia niespecyficznej amplifikacji, zapobiegania tworzeniu się dimerów starterów i zwiększenia wydajności produktu.

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