71340-M

pCDFDuet-1 DNA - Novagen

• Synonim(y): Duet-1 DNA, Novagen DNA Vector, pCDFDuet-1 DNA
• Kod UNSPSC: 41106602
• NACRES: NA.51

Właściwości

• Postać: liquid
• Poziom jakości: 100
• producent / nazwa handlowa: Novagen^®
• warunki przechowywania: OK to freeze
• Promotor: Promoter name: T7
• selection: spectinomycin
• temp. przechowywania: −70°C

Opis

Opis ogólny

pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet-1, pACYCDuet-1, and pRSFDuet-1 or pCOLADuet-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.

Zastosowanie

pCDFDuet-1 DNA has been used as a vector for the expression of recombinant proteins in E-coli cells.

Ostrzeżenie

Toxicity: Standard Handling (A)

Informacje prawne

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable