5.30650

Luciferase Substrate, CycLuc1

• Synonim(y): Luciferase Substrate, CycLuc1, 2-(6,7-dihydro-5h-thiazolo[4,5-f]indol-2-yl)-4,5-dihydrothiazole-4-carboxylicacid
• Wzór empiryczny (zapis Hilla): C₁₃H₁₁N₃O₂S₂
• Masa cząsteczkowa: 305.38
• Kod UNSPSC: 12352200
• NACRES: NA.77

Właściwości

• Próba: ≥94% (HPLC)
• Poziom jakości: 100
• Postać: powder
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; protect from light
• kolor: yellow
• rozpuszczalność: DMSO: 100 mg/mL
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

A synthetic cyclic alkylamino luciferin that exhibitsproperties that are superior to those of D-luciferin. Binds to luciferase with higheraffinity and emits about 5.7-fold more light than aminoluciferin and3.2-fold more light than D-luciferin. Its superior light output is maintainedover a wide range (1-100 µM). Shown to readily cross the blood-brain barrier inmice and provides much improved noninvasive bioluminescence imaging (BLI) ofthe brain at a lower dose when compared to D-luciferin. Yields about 10-foldhigher bioluminescent signal than D-luciferin when Luc2 luciferase expressing4T1 breast cancer cells are implanted into the mammary fat pads of BABL/c mice.A rapid (4-5 min), long-lasting, and steady signal peak is observed followingan i.v injection of CycLuc1 in mice.

Zastosowanie

Luciferase Substrate, CycLuc1 has been used for transfecting HEK293 cells to study the inhibition of intracellular luciferase crystallization by D-luciferin and its synthetic analogues.

Działania biochem./fizjol.

Cell permeable: yes

Primary Target
Luciferase

Opakowanie

Packaged under inert gas

Ostrzeżenie

Toxicity: Standard Handling (A)

Inne uwagi

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable