517310
Anti-P-Glycoprotein Mouse mAb (C219)
liquid, clone C219, Calbiochem®
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About This Item
Kod UNSPSC:
12352203
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
C219, monoclonal
Formularz
liquid
zawiera
≤0.1% sodium azide as preservative
reaktywność gatunkowa
rat, hamster, human, mouse
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
avoid repeated freeze/thaw cycles
izotyp
IgG1
Warunki transportu
wet ice
temp. przechowywania
−20°C
informacje o genach
human ... ABCB1(5243)
Opis ogólny
Purified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
This Anti-P-Glycoprotein Mouse mAb (C219) is validated for use in FC, Frozen Sections, Immunoblotting, ICC, IP, Paraffin Sections for the detection of P-Glycoprotein.
Immunogen
Hamster and Human
SDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a multidrug resistant human cell line
Zastosowanie
Flow Cytometry (5-10 µg/1 x 10⁶ cells)
Frozen Sections (5-10 µg/ml)
Immunoblotting (1-10 µg/ml, see application references, see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (1-10 µg/ml)
Frozen Sections (5-10 µg/ml)
Immunoblotting (1-10 µg/ml, see application references, see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (1-10 µg/ml)
Opakowanie
Please refer to vial label for lot-specific concentration.
Ostrzeżenie
Toxicity: Standard Handling (A)
Postać fizyczna
In PBS, 1% BSA.
Rekonstytucja
Following initial thaw, aliquot and freeze (-20°C).
Komentarz do analizy
Positive Control
Cell lines or frozen tissue, such as liver or colon
Cell lines or frozen tissue, such as liver or colon
Inne uwagi
Also recognizes ~150 kDa sister P-glycoprotein (SPGP). Frozen sections should be fixed in acetone for 10 min at -20°C. Formalin-fixed, paraffin tissue sections should be deparaffinized and rehydrated by conventional means. Boiling samples may reduce visibility of the protein by immunoblotting (see application references). This antibody is also reported to work for immunocytochemistry and immunoprecipitation. A ~200 kDa protein that migrates in the same position as myosin is also seen by immunoblotting. Variables associated with assay conditions will dictate the proper working dilutions.
Beck, W.T., et al. 1996. Cancer Res. 56, 3010.
Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
Informacje prawne
ImmunoblottingLee, G., et al. 2001. J. Pharmacol. Exp. Ther.299, 204.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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