239807

Cyclopamine-KAAD

≥70% (sum of two isomers, HPLC), liquid, Hedgehog signaling inhibitor, Calbiochem^®

• Synonim(y): 3- Keto-N-( aminoetylo- aminokaproilo-dihydrocynamoilo)cyklopamina
• Kod UNSPSC: 12352116
• NACRES: NA.77

Właściwości

• Nazwa produktu: Cyclopamine-KAAD, InSolution, ≥70%, inhibits Hedgehog (Hh) signaling
• Poziom jakości: 100
• Próba: ≥70% (sum of two isomers, HPLC)
• Formularz: liquid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• Warunki transportu: dry ice
• temp. przechowywania: −70°C

Opis

Opis ogólny

A cell-permeable potent analog of Cyclopamine (Cat. No. 239803) that specifically inhibits Hedgehog (Hh) signaling with similar or lower toxicity (IC₅₀ = 20 nM in Shh-LIGHT2 assay; 50 nM in p2^Ptch-/-cells; 500 nM in SmoA1-LIGHT cells). Binds to SmoA1 and promotes its exit from the endoplasmic reticulum. Suppresses both ShhNp-induced pathway activity and SmoA1-induced reporter activity. Reported to cause regression of murine tumor allografts in vivo and induce rapid cell death in human medulloblastoma. Shown to sensitize human glioma cells to TRAIL-induced apoptosis. The solid form of this compound (Cat. No. 239803) is also available.

Działania biochem./fizjol.

Cell permeable: yes

Primary Target
SmoA1

Opakowanie

Packaged under inert gas

Ostrzeżenie

Toxicity: Irritant (B)

Postać fizyczna

A 1 mM (50 µg/72 µL) solution of Cyclopamine-KAAD (Cat. No. 239804) in DMSO.

Rekonstytucja

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C.

Inne uwagi

Siegelin, M.D. et al. 2009. Neurobiol. Dis.34, 259.
Watkins, D.N., et al. 2003. Nature422, 313.
Berman, D.M., et al. 2002. Science297, 1559.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.
Chen, J.K., et al. 2002. Genes Dev.16, 2743.
Frank-Kamenetsky, M., et al. 2002. J. Biol.1, 10.
Taipale, J., et al. 2000. Nature406, 1005.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): 188.6 °F - closed cup - (refers to pure substance)
• Temperatura zapłonu (°C): 87 °C - closed cup - (refers to pure substance)