219402

Cathepsin L, Human Liver

Cathepsin L, Human Liver, CAS 60616-82-2, is a native, the most potent of all the lysosomal proteinases. Plays a major role in the proteolysis of both cellular and endocytosed macromolecules.

• Synonim(y): CTSL, CTSL1, Human Cathepsin L, Major excreted protein (MEP)
• Numer EC enzymu: 3.4.22.15 (BRENDA, IUBMB)
• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• pochodzenie biologiczne: human liver
• Poziom jakości: 100
• Postać: liquid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze; avoid repeated freeze/thaw cycles
• metody: activity assay: suitable
• przydatność: suitable for molecular biology
• Zastosowanie: life science and biopharma
• Warunki transportu: wet ice
• temp. przechowywania: −70°C
• informacje o genach: human ... CTSL(1514)

Opis

Opis ogólny

Research area: Cell Signaling
Cathepsin L, Human Liver, native, is the most potent of all the lysosomal proteinases. Cathepsin L (CTSL) belongs to the papain subfamily of cysteine proteases and is mainly located in endolysosomal vesicles.

Działania biochem./fizjol.

Cathepsin L (CTSL) has higher activity than cathepsins B and H in the degradation of a variety of physiological protein substrates. It is believed to be responsible for the generation of endostatin from the NC1 domain in collagen XVII. It is responsible for regulating cell cycle, nucleotide-binding domain, leucine-rich–containing family, pyrin domain–containing-3 (NLRP3) inflammasome activation, proteolytic processing of Bid during apoptosis, and TGF-β signaling. It can degrade a wide range of proteins, encompassing enzymes, receptors, and transcription factors. Additionally, it produces active enzymes, receptors, and biologically active peptides through controlled proteolysis. Moreover, Cathepsin L has been observed to enhance tumor cell migration by lowering cell-cell adhesion and breaking down elements of the extracellular matrix. The expression of CTSL escalates in several types of cancers, including glioma, melanoma, pancreatic, prostate, and breast cancer. It plays a major role in the proteolysis of both cellular and endocytosed macromolecules.

Opakowanie

Please refer to vial label for lot-specific concentration.

Ostrzeżenie

Toxicity: Standard Handling (A)

Definicja jednostki

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Z-FR-AFC per min at 25°C, pH 5.5.

Postać fizyczna

In 400 mM NaCl, 20 mM malonate buffer, 1 mM EDTA, pH 5.5.

Uwaga dotycząca przygotowania

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Rekonstytucja

Following initial thaw, aliquot and freeze (-70°C).

Inne uwagi

Note: 1 mU = 1 milliunit

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2