16-219
Anti-Myc Tag Antibody, clone 4A6, agarose conjugate
clone 4A6, Upstate®, from mouse
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
4A6, monoclonal
reaktywność gatunkowa
human
producent / nazwa handlowa
Upstate®
metody
immunoprecipitation (IP): suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... MYC(4609)
Opis ogólny
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Specyficzność
Reactivity with other species has not been confirmed.
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc.
Zastosowanie
Anti-Myc Tag Antibody, clone 4A6, agarose conjugate detects level of Myc Tag & has been published & validated for use in IP.
Immunoaffinity Purification:
A previous lot was used to purify Myc-tagged proteins. Elute with 100 mM tetraethyl ammonium (TEA), pH 11.5.
A previous lot was used to purify Myc-tagged proteins. Elute with 100 mM tetraethyl ammonium (TEA), pH 11.5.
Cechy i korzyści
Format: Gel Immobilized
Jakość
Routinely evaluated by immunoprecipitation of Myc-tagged Akt/PKB from a COS transfected cell lysate, subsequent detection using anti-Myc Tag (05-724).
Immunoprecipitation Analysis:
4 μg of anti-Myc Tag, clone 4A6, agarose conjugate immunoprecipitated Myc-tagged Akt/PKB from 250 μg of a COS transfected cell lysate, which was then detected by immunoblot analysis, using 0.5 μg/mL of anti-Myc Tag, clone 4A6 (Catalog # 05-724).
Immunoprecipitation Analysis:
4 μg of anti-Myc Tag, clone 4A6, agarose conjugate immunoprecipitated Myc-tagged Akt/PKB from 250 μg of a COS transfected cell lysate, which was then detected by immunoblot analysis, using 0.5 μg/mL of anti-Myc Tag, clone 4A6 (Catalog # 05-724).
Opis wartości docelowych
Varies
Postać fizyczna
Purified mouse monoclonal IgG1 covalently coupled to protein G agarose beads and provided as a 50% slurry in buffer containing PBS, pH 7.4, and 0.05% sodium azide. Liquid suspension.
Przechowywanie i stabilność
Stable for 1 year at 2-8ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Komentarz do analizy
Control
Cells transfected with Myc-tagged fusion vector.
Cells transfected with Myc-tagged fusion vector.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
X Fu et al.
Journal of dental research, 96(12), 1451-1458 (2017-07-22)
Previous studies have identified the odd-skipped related 2 (Osr2) transcription factor as a key intrinsic regulator of palatal shelf growth and morphogenesis. However, little is known about the molecular program acting downstream of Osr2 in the regulation of palatogenesis. In
Mutagenesis and analysis of genetic mutations in the GC-rich KISS1 receptor sequence identified in humans with reproductive disorders.
Luciana Madeira da Silva,Lauren Vandepas,Suzy D C Bianco
Journal of Visualized Experiments null
Yadong Xu et al.
The Prostate, 79(6), 657-666 (2019-02-05)
DHX15 is a member of the DEAH-box (DHX) RNA helicase family. Our previous study identified it as an AR coactivator which contributes to prostate cancer progression. We investigated DHX15 expression in castration resistant prostate cancer specimens and the influence of
Identification of a subunit of a novel Kleisin-beta/SMC complex as a potential substrate of protein phosphatase 2A
Yeong, F. M., et al
Current Biology, 13, 2058-2064 (2003)
Jingyue Xu et al.
PloS one, 11(7), e0159597-e0159597 (2016-07-22)
Renal hypoplasia is a common cause of pediatric renal failure and several adult-onset diseases. Recent studies have associated a variant of the OSR1 gene with reduction of newborn kidney size and function in heterozygotes and neonatal lethality with kidney defects
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej