16-101
Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
clone 4G10®, Upstate®, from mouse
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
4G10®, monoclonal
reaktywność gatunkowa
vertebrates
producent / nazwa handlowa
Upstate®
metody
immunoprecipitation (IP): suitable
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... PID1(55022)
Opis ogólny
Anti-Phosphotyrosine antibody (05-321), cross-linked to protein A agarose by dimethylpimelimidate
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Specyficzność
Recognizes tyrosine-phosphorylated proteins from all species.
Immunogen
Phosphotyramine-KLH.
Zastosowanie
Anti-Phosphotyrosine Antibody, clone 4G10, agarose conjugate is an antibody against Phosphotyrosine for use in IP.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
This product is useful for immunoprecipitation and immunoaffinity chromatography methods.
Suitable for immunoprecipitation and affinity purification.
Suitable for immunoprecipitation and affinity purification.
Cechy i korzyści
Format: Gel Immobilized
Jakość
Evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.
Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Opis wartości docelowych
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Postać fizyczna
1 mg of Anti-Phosphotyrosine monoclonal antibody covalently coupled to 1 mL of protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.05% Kathon as a preservative, for a total volume of 2 mL. Liquid suspension.
Protein A purified
Przechowywanie i stabilność
Stable for 1 year at 2-8ºC from date of receipt.
Komentarz do analizy
Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Identification of oncogenic point mutations and hyperphosphorylation of anaplastic lymphoma kinase in lung cancer.
Wang, YW; Tu, PH; Lin, KT; Lin, SC; Ko, JY; Jou, YS
Neoplasia null
Activation of src-related tyrosine kinases by IL-3
Anderson, S. M. and Jorgensen, B.
Journal of immunology (Baltimore, Md. : 1950), 155, 1660-1670 (1995)
Robin White et al.
The Journal of biological chemistry, 287(3), 1742-1754 (2011-12-01)
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Sakata, H, et al.
The Journal of Biological Chemistry, 272, 9457-9463 (1997)
Phosphorylation site dynamics of early T-cell receptor signaling.
Chylek, LA; Akimov, V; Dengjel, J; Rigbolt, KT; Hu, B; Hlavacek, WS; Blagoev, B
Testing null
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