16-105
Przeciwciało przeciw fosfotyrozynie, klon 11971, koniugat HRP
clone 4G10®, Upstate®, from mouse
Synonim(y):
Przeciwciało przeciwko fosfotyrozynie
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.56
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
peroxidase conjugate
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
4G10®, monoclonal
reaktywność gatunkowa (przewidywana na podstawie homologii)
all
producent / nazwa handlowa
Upstate®
metody
ELISA: suitable
western blot: suitable
izotyp
IgG2bκ
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
phosphorylation (pTyr)
informacje o genach
human ... PID1(55022)
Opis ogólny
Anti-Phosphotyrosine antibody (05-321), cross-linked to horseradish peroxidase
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.
Description
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10. Purified by Protein G-Sepharose chromatography and cross-linked to horseradish peroxidase.
Description
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10. Purified by Protein G-Sepharose chromatography and cross-linked to horseradish peroxidase.
Specyficzność
Recognizes tyrosine-phosphorylated proteins from all species.
Immunogen
Phosphotyramine-KLH.
Zastosowanie
Anti-Phosphotyrosine Antibody, clone 4G10, HRP conjugate detects level of Phosphotyrosine & has been published & validated for use in ELISA & WB.
For use in applications in which an HRP conjugate is advantageous, such as WB and IP.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Jakość
Routinely evaluated in western blot on RIPA lysates from EGF-treated human A431 carcinoma cells.
Western Blot Analysis:
Tested at 0.5-2 μg/mL, with optimal performance at 2 μg/mL (10 μL per 10 mL blocking buffer). This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human A431 carcinoma cells.
Western Blot Analysis:
Tested at 0.5-2 μg/mL, with optimal performance at 2 μg/mL (10 μL per 10 mL blocking buffer). This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human A431 carcinoma cells.
Opis wartości docelowych
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Postać fizyczna
200 μg of IgG2bκ-HRP conjugate in 100 μL PBS containing 0.05% Kathon® . Sufficient for 10 western blots, if used at the optimum concentration of 2 μg/mL in 10 mL blocking buffer. Liquid.
Protein G-Sepharose Chromatography
Przechowywanie i stabilność
Stable for 6 months at 2-8°C from date of receipt. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
NOTE: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
NOTE: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
Komentarz do analizy
Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Informacje prawne
4G10 is a registered trademark of Upstate Group, Inc.
Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Ta strona może zawierać tekst przetłumaczony maszynowo.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Aquatic Chronic 2 - Skin Sens. 1
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Valerie Chew et al.
The Journal of biological chemistry, 282(37), 27181-27191 (2007-07-21)
The role of the paxillin superfamily of adaptor proteins in B cell antigen receptor (BCR) signaling has not been studied previously. We show here that leupaxin (LPXN), a member of this family, was tyrosine-phosphorylated and recruited to the plasma membrane
Jason E Hudak et al.
Nature chemical biology, 10(1), 69-75 (2013-12-03)
The increase of cell surface sialic acid is a characteristic shared by many tumor types. A correlation between hypersialylation and immunoprotection has been observed, but few hypotheses have provided a mechanistic understanding of this immunosuppressive phenomenon. Here, we show that
Gajanan Sathe et al.
Clinical proteomics, 13, 13-13 (2016-06-17)
Curcumin, derived from the rhizome Curcuma longa, is a natural anti-cancer agent and has been shown to inhibit proliferation and survival of tumor cells. Although the anti-cancer effects of curcumin are well established, detailed understanding of the signaling pathways altered
Antagonistic interplay between necdin and Bmi1 controls proliferation of neural precursor cells in the embryonic mouse neocortex.
Minamide, R; Fujiwara, K; Hasegawa, K; Yoshikawa, K
Testing null
Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL.
Fan, A; Wu, X; Wu, H; Li, L; Huang, R; Zhu, Y; Qiu, Y; Fu, J; Ren, J; Zhu, C
Testing null
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej