07-432
Anti-K+/Cl- Cotransporter (KCC2) Antibody
Upstate®, from rabbit
Synonim(y):
Electroneutral potassium-chloride cotransporter 2, Erythroid K-Cl cotransporter 2, Neuronal K-Cl cotransporter, electroneural potassium-chloride cotransporter 2, solute carrier family 12 (potassium-chloride transporter), member 5
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
rat, canine, dog
producent / nazwa handlowa
Upstate®
metody
immunocytochemistry: suitable
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
dog ... Slc12A5(485905)
rat ... Slc12A5(171373)
Opis ogólny
KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporters. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA′s inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
Specyficzność
Recognizes rat K+/Cl- Cotransporter (KCC2), Mr ~140 kDa. An unknown band >200 kDa was also detected.
Wide range of species cross-reactivity expected due to sequence homology.
Immunogen
N-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2).
Zastosowanie
Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect K+/Cl- Cotransporter (KCC2) in Madin Darby Canine Kidney (MDCK) cells at 1:200 dilution.
This antibody has been reported by an independent laboratory to detect K+/Cl- Cotransporter (KCC2) in Madin Darby Canine Kidney (MDCK) cells at 1:200 dilution.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
This Anti-K+/Cl-Cotransporter (KCC2) Antibody is validated for use in IC, WB for the detection of K+/Cl-Cotransporter (KCC2) with more than 15 product citations.
Jakość
Routinely evaluated by western blot in rat brain membrane preperations.
Western Blot Analysis: 1.0 µg/mL of this lot detected K+/Cl- Cotransporter (KCC2) in rat brain membrane preparations.
Western Blot Analysis: 1.0 µg/mL of this lot detected K+/Cl- Cotransporter (KCC2) in rat brain membrane preparations.
Opis wartości docelowych
140 kDa
Postać fizyczna
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol. Liquid at -20°C.
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Komentarz do analizy
Control
Rat brain membrane preparation.
Rat brain membrane preparation.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
A Stil et al.
Neuroscience, 164(2), 809-821 (2009-08-25)
The classical GABA/glycine hyperpolarizing inhibition is not observed in the immature spinal cord. GABA(A) and glycine receptors are anions channels and the efficacy of inhibitory transmission in the spinal cord is largely determined by the gradient between intracellular and extracellular
Effects of third trimester-equivalent ethanol exposure on Cl(-) co-transporter expression, network activity, and GABAergic transmission in the CA3 hippocampal region of neonatal rats.
Everett, JC; Licon-Mu?oz, Y; Valenzuela, CF
Alcohol null
J R Williams et al.
The Journal of biological chemistry, 274(18), 12656-12664 (1999-04-23)
The neuron-specific K-Cl cotransporter (KCC2) is hypothesized to function as an active Cl- extrusion pathway important in postsynaptic inhibition mediated by ligand-gated anion channels, like gamma-aminobutyric acid type A (GABAA) and glycine receptors. To understand better the functional role of
MicroRNA-92 modulates K(+) Cl(-) co-transporter KCC2 expression in cerebellar granule neurons.
Barbato, Christian, et al.
Journal of Neurochemistry, 113, 591-600 (2010)
T Q Vu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 20(4), 1414-1423 (2000-02-09)
The processing of signals by integrative neurons in the retina and CNS relies strongly on inhibitory synaptic inputs, principally from GABAergic and glycinergic neurons that serve primarily to hyperpolarize postsynaptic neurons. Recent evidence indicates that the neuron-specific K-Cl cotransporter 2
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