07-428-I

Anti-phospho eNOS Antibody (Ser1177)

from rabbit, purified by affinity chromatography

• Synonim(y): Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• reaktywność gatunkowa (przewidywana na podstawie homologii): bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), porcine (based on 100% sequence homology), sheep (based on 100% sequence homology), canine (based on 100% sequence homology)
• metody: western blot: suitable
• numer dostępu NCBI: NP_000594
• numer dostępu UniProt: P29474
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pSer1177)
• informacje o genach: human ... NOS3(4846)

Opis

Opis ogólny

NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human eNOS phosphorylated at Ser1177.

Zastosowanie

Anti-phospho eNOS Antibody (Ser1177) is a highly specific rabbit polyclonal antibody, that targets eNOS/NOS III & has been tested in western blotting.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Jakość

Evaluated by Western Blotting in untreated and VEGF treated HUVEC cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected phospho eNOS (Ser1177) in 10 µg of VEGF treated HUVEC cell lysate.

Opis wartości docelowych

~133 kDa observed

Powiązanie

Replaces: 07-428

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable