07-209-I

Anti-phospho-SHC Antibody (Tyr239, Tyr240)

from rabbit, purified by affinity chromatography

• Synonim(y): p46Shc (pTyr194/pTyr195), p52Shc (pTyr239/pTyr240), p66Shc (pTyr349/pTyr350), SHC-transforming protein 1, tyrosine-phosphorylated, SH2 domain protein C1, tyrosine-phosphorylated, SHC-transforming protein 3, tyrosine-phosphorylated, SHC-transforming prote
• Kod UNSPSC: 12352203
• eCl@ss: 32160702

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• reaktywność gatunkowa (przewidywana na podstawie homologii): mouse (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology), chicken (based on 100% sequence homology)
• metody: western blot: suitable
• numer dostępu NCBI: NP_892113
• numer dostępu UniProt: P29353
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: phosphorylation (pTyr239/pTyr240)
• informacje o genach: human ... SHC1(6464)

Opis

Opis ogólny

SHC-transforming protein 1 (UniProt P29353; also known as SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID 6464) in human. Originally identified as an SH2-containing proto-oncogene involved in growth factor signaling, Shc proteins function as adaptor molecules recruited by numerous receptors (e.g. integrins, GPCRs, and receptors for growth factors, antigens, cytokines, and hormones) for signling. In addition to the ubiquitously expressed Shc (a.k.a. ShcA by SHC1/SHCA gene), there exist also ShcB (by SHC2/SHCB gene) and ShcC (by SHC3/SHCC gene), which are predominantly expressed in neuronal cells. Alternative splicings result in six ShcA isoforms, including p66Shc, p52Shc, and p46Shc. All ShcA isoforms possess two distinct domains, PTB (a.a. 156-339) and SH2 (a.a. 488-579), that bind phosphotyrosine-containing sequences, as well as a central region (CH1; a.a. 340-487) that contains critical tyrosine phosphorylation sites. The phosphotyrosine-binding (PTB) domain is also referred to as phosphotyrosine-interaction domain or PID.

Specyficzność

Numbering of the target pTyr residues varies depending on the species and spliced isoforms. Target pTyr residues this rabbit polyclonal detects correspond to pTyr349/pTyr350 of human spliced isoform 6 and p66Shc, pTyr239/pTyr240 of human spliced isoform 7 and p52Shc, pTyr194/pTyr195 of isoform p46Shc, and pTyr135/pTyr136 of isoform 5 reported by UniProt (P29353).

Immunogen

KLH-conjugated linear peptide corresponding to a target site sequence surrounding phosphorylated Tyr239 and Tyr240 of human spliced isoform p52Shc.

Zastosowanie

Detect SHC tyr239 and tyr240 phosphorylation using this rabbit polyclonal Anti-phospho-SHC (Tyr239, Tyr240) antiboody, Cat. No. 07-209-I, validated for use in Western Blotting.

Jakość

Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected SHC tyrosine phosphorylation induction in 10 µg of lysate from EGF-stimulated HEK293 cells.

Opis wartości docelowych

~52 kDa observed. 62.82/51.61/46.67/40.42/62.89/51.68 kDa (human isoform p66Shc/p52Shc/p46Shc/5/6/7), 62.61/51.41/46.47 kDa (mouse isoform p66Shc/p52Shc/p47Shc), and 51.50 kDa (rat) calculated.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable