07-1719

Anti-FOXO3A Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, forkhead box O3, forkhead box O3A, forkhead homolog (rhabdomyosarcoma) like 1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• reaktywność gatunkowa (przewidywana na podstawie homologii): mouse
• metody: immunocytochemistry: suitable; western blot: suitable
• numer dostępu NCBI: NP_001446
• numer dostępu UniProt: O43524
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... FOXO3(2309)

Opis

Opis ogólny

Forkhead box O3A (FOXO3A) is a member of the forkhead family of transcription factors (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. Upon oxidative stress, FOXO3A translocates to the nucleus where binding to the DNA sequence 5’-[AG]TAAA[TC]A-3’ occurs and IGF-1 is dephosphlorated, resulting in apoptosis. In order for the cell to survive, IGF-1 must be phosphlorated by AKT1/PKB at Thr32 and Ser-253, which will result in retention and inactivation of FOXO3A in the cytoplasm. Translocation of FOXO3A with the MLL gene has been associated with secondary acute leukemia.

Specyficzność

This antibody recognizes FOXO3A.

Immunogen

Synthetic peptide corresponding to FOX03A

Zastosowanie

Immunocytochemistry:
A 1:500 dilution of this antibody was used to detect FOXO3A in HeLa cells. Positive staining in the nucleus.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Transcription Factors

This Anti-FOXO3A Antibody is validated for use in WB, IC for the detection of FOXO3A.

Jakość

Western Blot Analysis:
1:500 dilution of this antibody was used to detect FOXO3A in Jurkat cell lysate.

Opis wartości docelowych

~80 kDa

Powiązanie

Replaces: 04-1007

Postać fizyczna

Affinity purified

Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Przechowywanie i stabilność

Stable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.

Komentarz do analizy

Control
FOXO3A in Jurkat cell lysate.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable