07-1404

Anti-GLUT-4 Antibody, C-terminus

from rabbit, purified by affinity chromatography

• Synonim(y): Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive, GLUT-4
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: mouse, human
• reaktywność gatunkowa (przewidywana na podstawie homologii): rat (based on 100% sequence homology)
• metody: immunohistochemistry: suitable; western blot: suitable
• numer dostępu NCBI: NP_001033
• numer dostępu UniProt: P14672
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... SLC2A4(6517)

Opis

Opis ogólny

The protein called GLUT-4, or more correctly Solute carrier family 2, facilitated glucose transporter member 4 and encoded by the human gene SLC2A4/GLUT4 is a primary insulin regulated facilitative glucose transporter found primarily in skeletal and cardiac muscle, as well as brown and white fat but is also expressed in other tissues such as brain. In the brain GLUT4 is found exclusively in neurons. GLUT-4 is facilitative because it is not always present on the cell plasma membrane. GLUT-4 only localizes to the plasma membrane upon insulin induction. GLUT-4 is localized to the perinuclear region in resting cells. Mutations in GLUT-4 are a cause of one form of non-insulin dependent diabetes mellitus. GLUT4 function as well as its transcriptional regulation is highly conserved throughout the vertebrate species.

Specyficzność

Glucose transporter 4 (GLUT-4) is a protein present in rat skeletal muscle, heart and adipose tissues. GLUT-4 is highly conserved between species, and the peptide sequence selected for production of this antibody is identical in rat, human (amino acids 498-510), and mouse. This antibody shows no cross-reactivity of HepG2 type transporter from rat brain or other glucose transporters.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human GLUT-4.

Zastosowanie

Detect GLUT-4 using this Anti-GLUT-4 Antibody, C-terminus validated for use in WB, IH(P), ELISA.

Immunohistochemistry Analysis: A 1:50-2,000 dilution from a representative lot detected GLUT-4 in human cardiac myocytes and human skeletal muscle myocytes.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Jakość

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GLUT-4 in 10 µg of NIH3T3 cell lysate.

Opis wartości docelowych

~58 kDa observed

Powiązanie

Replaces: AB1346

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
NIH3T3 cell lysate

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2