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07-1364

Sigma-Aldrich

Anti-ECT2 Antibody

from rabbit, purified by affinity chromatography

Synonim(y):

epithelial cell transforming sequence 2 oncogene, Epithelial cell-transforming sequence 2 oncogene, protein ECT2

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

100

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

oczyszczone przez

affinity chromatography

reaktywność gatunkowa

pig, human

reaktywność gatunkowa (przewidywana na podstawie homologii)

porcine (based on 100% sequence homology), rabbit (based on 100% sequence homology), monkey (based on 100% sequence homology), opossum (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)

metody

immunocytochemistry: suitable
western blot: suitable

numer dostępu UniProt

Q9H8V3

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... ECT2(1894)

Opis ogólny

Epithelial cell-transforming sequence 2 oncogene (ECT2) is a guanine nucleotide exchange factor or GEF that belongs to the Dbl family of Rho GEFs. ECT2 is composed of 2 BRCT domains within the N-terminus, a DBL-homology domain, and a PH domain. Studies have shown ECT2 to localize to both the cell cortex and central spindles, and that it is a crucial part of cleavage furrow formation during cytokenises. Overexpression of cleaved ECT2, containing only the N-terminal domain, has been shown to cause multinucleation. As a GEF it has been shown to bind specifically to Rac proteins, RhoA, and RhoC.

Specyficzność

This antibody recognizes ECT2 at the
C-terminus.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to human ECT2 at the C-terminus.

Zastosowanie

Anti-ECT2 Antibody is an antibody against ECT2 for use in WB & IC.
Research Category
Signaling
Research Sub Category
G-proteins
Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected ECT2 in A549 cell lysate.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory on Heya8 cells. (Der Channing, University of North Carolina at Chapel Hill, NC, channing_der@med.unc.edu)

Jakość

Evaluated by Western Blot in A549 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected ECT2 on 10 µg of A549 cell lysate.

Opis wartości docelowych

~ 105 kDa observed

Postać fizyczna

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
A549 cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Danielle R Cook et al.
Genes & cancer, 2(10), 932-942 (2012-06-16)
Ect2 is a member of the human Dbl family of guanine nucleotide exchange factors (RhoGEFs) that serve as activators of Rho family small GTPases. Although Ect2 is one of at least 25 RhoGEFs that can activate the RhoA small GTPase
Ming Yi et al.
Experimental hematology & oncology, 11(1), 107-107 (2022-12-27)
Breast cancer is the most common malignancy and the second leading cause of cancer-related death in women. Recent studies have indicated that aberrant activation of Rho GTPases relates to the malignant properties of breast cancer cells. As the guanine nucleotide
Laurent Guillemot et al.
Molecular biology of the cell, 25(13), 1995-2005 (2014-05-09)
The regulation of Rho-family GTPases is crucial to direct the formation of cell-cell junctions and tissue barriers. Cingulin (CGN) and paracingulin (CGNL1) control RhoA activation in epithelial cells by interacting with RhoA guanidine exchange factors. CGNL1 depletion also inhibits Rac1
Bin Liu et al.
Translational oncology, 11(3), 700-714 (2018-04-10)
Pancreatic cancer is the fourth leading cause for cancer-related death, and early diagnosis is one key to improve the survival rate of this disease. Molecular biomarkers are an important method for diagnostic use in pancreatic cancer. We used data from
Yi Liu et al.
Cell reports, 30(3), 771-782 (2020-01-23)
Lung squamous cell carcinoma (LSCC) is a prevalent form of lung cancer exhibiting distinctive histological and genetic characteristics. Chromosome 3q26 copy number gain (CNG) is a genetic hallmark of LSCC present in >90% of tumors. We report that 3q26 CNGs
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