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Key Documents

05-338

Sigma-Aldrich

Anti-Fas Antibody (human, neutralizing), clone ZB4

clone ZB4, Upstate®, from mouse

Synonim(y):

APO-1 cell surface antigen, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, CD95 antigen, FASLG receptor, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, apoptosis antigen 1, tumor necrosis factor receptor superfamily member 6, tu

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

ZB4, monoclonal

reaktywność gatunkowa

human

producent / nazwa handlowa

Upstate®

metody

flow cytometry: suitable
neutralization: suitable
western blot: suitable

izotyp

IgG1

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

dry ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... FAS(355)

Opis ogólny

Fas (also known as Apo-1 and CD95) is a 40-50 kDa cell surface glycoprotein and a member of the TNF receptor family. Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell. The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD. FADD facilitates binding of caspase-8. which is activated to induce caspase-8 mediated apoptosis. Fas also plays a role in T cell mediated cytotoxicity and T cell development. Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes. The antagonistic ZB4 clone anti-Fas monoclonal has been used to block the apoptosis-inducing activity of the Anti-Fas CH-11 clone in multiple publications.
This product does not induce apoptosis in cell culture.

Specyficzność

This antibody does not recognize TNF and mouse Fas.
This antibody recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. It does not induce apoptosis in cell culture.

Immunogen

Recombinant human Fas.

Zastosowanie

Anti-Fas Antibody (human, neutralizing), clone ZB4 detects level of Fas & has been published & validated for use in WB, FC, NEUT.
Flow Cytometry: A previous lot of this antibody used 5-20 μg/mL. Western Blot: A previous lot of this antibody used 5 μg/mL.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Jakość

Evaluated by neutralization of apoptosis induced by anti-Fas (human, activating), clone CH11 (Catalog # 05-201) treatment of human Jurkat cells.

Neutralization: 10-500 ng/mL of this antibody was incubated for one hour with human Fas-transfected cells and inhibited the anti-Fas (human, activating), clone CH11 (Catalog # 05-201). Cell viability was assessed using a WST-1 assay

Opis wartości docelowych

45 kDa

Postać fizyczna

Ammonium sulfate and purified mouse monoclonal IgG1 in PBS containing 50% glycerol. Store at -20°C.
Format: Purified
Protein A purified

Przechowywanie i stabilność

Stable for 1 year at from date of receipt.

Komentarz do analizy

Control
Various human cells

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.

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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Involvement of apoptosis antigen Fas in clonal deletion of human thymocytes
Yonehara, S, et al
International Immunology, 6, 1849-1856 (1994)
Emilie P Buddingh et al.
Cancer immunology, immunotherapy : CII, 60(4), 575-586 (2011-01-18)
High-grade osteosarcoma occurs predominantly in adolescents and young adults and has an overall survival rate of about 60%, despite chemotherapy and surgery. Therefore, novel treatment modalities are needed to prevent or treat recurrent disease. Natural killer (NK) cells are lymphocytes
Networked T cell death following macrophage infection by Mycobacterium tuberculosis.
Macdonald, SH; Woodward, E; Coleman, MM; Dorris, ER; Nadarajan, P; Chew, WM; McLaughlin et al.
Testing null
E Lacana et al.
Nature medicine, 5(5), 542-547 (1999-05-06)
Programmed cell death is a process required for the normal development of an organism. One of the best understood apoptotic pathways occurs in T lymphocytes and is mediated by Fas/Fas ligand (FasL) interaction. During studies of apoptosis induced by T
Stimulated human lamina propria T cells manifest enhanced Fas-mediated apoptosis
Boirivant, M., et al
The Journal of Clinical Investigation, 98, 2616-2622 (1996)

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