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Key Documents

04-858

Sigma-Aldrich

Anti-Histone H4 Antibody, pan, rabbit monoclonal

culture supernatant, clone 62-141-13, Upstate®

Synonim(y):

H4, Histone H4

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

forma przeciwciała

culture supernatant

rodzaj przeciwciała

primary antibodies

klon

62-141-13, monoclonal

reaktywność gatunkowa

Saccharomyces cerevisiae, human, vertebrates, chicken

producent / nazwa handlowa

Upstate®

metody

ChIP: suitable
multiplexing: suitable
western blot: suitable

izotyp

IgG

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

dry ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... H4C1(8359)

Opis ogólny

This antibody recognizes histone H4 independant of any post-translational modifications.

Specyficzność

Recognizes Histone H4.

Immunogen

Epitope: a.a. 25-28
Synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28

Zastosowanie

Anti-Histone H4 Antibody, pan is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex.
Immunoblot Analysis:
A 1:100,000 dilution of a previous lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated) (Figure B).

Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).

Multiplexing:
Serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the aminoterminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Jakość

Routinely evaluated by immunoblot using HeLa cell acid extract.

Opis wartości docelowych

10 kDa

Powiązanie

Replaces: 05-858

Postać fizyczna

Cultured supernantant in 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Komentarz do analizy

Control
Recombinant Histone H4 (Catalog # 14-412) or HeLa cell acid extract.

Informacje prawne

Luminex is a registered trademark of Luminex Corp
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Multiple forms of H4 histone mRNA in human cells.
Lichtler, A C, et al.
Proceedings of the National Academy of Sciences of the USA, 77, 1942-1946 (1980)
Soheila Dolatabadi et al.
Frontiers in oncology, 12, 816894-816894 (2022-02-22)
Myxoid liposarcoma is one of the most common sarcoma entities characterized by FET fusion oncogenes. Despite a generally favorable prognosis of myxoid liposarcoma, chemotherapy resistance remains a clinical problem. This cancer stem cell property is associated with JAK-STAT signaling, but
Peter J Thompson et al.
PLoS genetics, 11(1), e1004933-e1004933 (2015-01-23)
Retrotransposition of endogenous retroviruses (ERVs) poses a substantial threat to genome stability. Transcriptional silencing of a subset of these parasitic elements in early mouse embryonic and germ cell development is dependent upon the lysine methyltransferase SETDB1, which deposits H3K9 trimethylation
Venizelos Papayannopoulos et al.
The Journal of cell biology, 191(3), 677-691 (2010-10-27)
Neutrophils release decondensed chromatin termed neutrophil extracellular traps (NETs) to trap and kill pathogens extracellularly. Reactive oxygen species are required to initiate NET formation but the downstream molecular mechanism is unknown. We show that upon activation, neutrophil elastase (NE) escapes
Ryan W Logan et al.
Molecular psychiatry, 26(8), 4066-4084 (2020-11-26)
Valproate (VPA) has been used in the treatment of bipolar disorder since the 1990s. However, the therapeutic targets of VPA have remained elusive. Here we employ a preclinical model to identify the therapeutic targets of VPA. We find compounds that

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