05-1339
Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304
clone CMA304, from mouse
Synonim(y):
H3K4me3, Histone H3 (tri methyl K4), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
CMA304, monoclonal
reaktywność gatunkowa
human, vertebrates
metody
ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
multiplexing: suitable
western blot: suitable
izotyp
IgG1κ
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
trimethylation (Lys4)
informacje o genach
human ... H3C1(8350)
Opis ogólny
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H3 (Lys4) is is found at the 5′ end of active genes and contributes to transcriptional activation by recruiting chromatin-remodelling enzymes. Accordingly, the inactive X chromosome is largely devoid of this mark.
Specyficzność
Broad species cross-reactivity is expected, based on sequence homology.
This antibody specifically recognizes Histone H3 trimethylated at Lys4. The antibody binding specificity allows for modifications of Lys91.
Immunogen
Epitope: Trimethylated Lys4
Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, trimethylated on Lys4, conjugated to KLH.
Zastosowanie
Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304 is a mouse monoclonal antibody for detection of Trimethyl Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in ELISA, ICC, IP, WB, Mplex, DB.
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1
Multiplexing:
This antibody specifically recognizes histone H3 trimethylated on Lys4 by Luminex® assay.
This antibody has been shown by an outside laboratory to be suitable for ELISA.1
Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1
Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1
Multiplexing:
This antibody specifically recognizes histone H3 trimethylated on Lys4 by Luminex® assay.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Jakość
Western Blot Analysis:
0.5 - 2 μg/mL (1:500-2000) dilution of this antibody detected trimethyl Histone H3 (Lys4) on 10 μg of HeLa acid extract.
0.5 - 2 μg/mL (1:500-2000) dilution of this antibody detected trimethyl Histone H3 (Lys4) on 10 μg of HeLa acid extract.
Opis wartości docelowych
approx. 17 kDa
Powiązanie
Replaces: 04-791
Postać fizyczna
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Komentarz do analizy
Control
HeLa Acid extract lysate
HeLa Acid extract lysate
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
Luminex is a registered trademark of Luminex Corp
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Jente Ottenburghs et al.
Genome biology and evolution, 13(4) (2021-02-04)
As a highly diverse vertebrate class, bird species have adapted to various ecological systems. How this phenotypic diversity can be explained genetically is intensively debated and is likely grounded in differences in the genome content. Larger and more complex genomes
Arginine methylation at histone H3R2 controls deposition of H3K4 trimethylation.
Kirmizis, Antonis, et al.
Nature, 449, 928-932 (2007)
AHT-ChIP-seq: a completely automated robotic protocol for high-throughput chromatin immunoprecipitation.
Aldridge, S; Watt, S; Quail, MA; Rayner, T; Lukk, M; Bimson, MF; Gaffney, D; Odom, DT
Genome Biology null
Maša Roller et al.
Genome biology, 22(1), 62-62 (2021-02-20)
To investigate the mechanisms driving regulatory evolution across tissues, we experimentally mapped promoters, enhancers, and gene expression in the liver, brain, muscle, and testis from ten diverse mammals. The regulatory landscape around genes included both tissue-shared and tissue-specific regulatory regions
Whole-genome mapping of histone H3 Lys4 and 27 trimethylations reveals distinct genomic compartments in human embryonic stem cells.
Zhao, Xiao Dong, et al.
Cell Stem Cell, 1, 286-298 (2007)
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