04-1565

Anti-NSD1 Antibody, clone 1NW-1A10

ascites fluid, clone 1NW-1A10, from mouse

• Synonim(y): Androgen receptor-associated coregulator 267, Lysine N-methyltransferase 3B, NR-binding SET domain-containing protein, Nuclear receptor-binding SET domain-containing protein 1, Sotos syndrome, nuclear receptor binding SET domain protein 1
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: ascites fluid
• rodzaj przeciwciała: primary antibodies
• klon: 1NW-1A10, monoclonal
• reaktywność gatunkowa: rat
• reaktywność gatunkowa (przewidywana na podstawie homologii): mouse (based on 100% sequence homology)
• metody: immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• izotyp: IgG1κ
• numer dostępu NCBI: NP_032765.3
• numer dostępu UniProt: O88491
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: mouse ... Nsd1(18193) ; rat ... Nsd1(306764)

Opis

Opis ogólny

NSD1 (also known as KMT3B) is a histone methyltransferase shown to methylate lysine 36 of histone H3 and lysine 20 of histone H4. NSD1 is a described as an intermediary transcription factor and depending on its cellular context, it is able to either negatively or positively influence transcription. Expression of this gene has been described in skeletal muscles, kidneys, adult and fetal brain, thymus and spleen. Heterozygous mutations and deletions of the NSD1 gene are associated with Sotos Syndrome, an autosomal dominant condition characterized by macrocephaly, tall stature, facial elongation, accelerated bone aging, and mental retardation.

Specyficzność

This antibody recognizes NSD1.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to mouse NSD1.

Zastosowanie

Anti-NSD1 Antibody, clone 1NW-1A10 is a mouse monoclonal antibody for detection of NSD1, also known as &rogen receptor-associated coregulator 267, Lysine N-methyltransferase 3B, has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Chromatin Biology

Used for immunohistochemistry on paraffin sections in Visser et al., (2012).
Used for fluorescent immunocytochemistry in Huang et al.,(1998).

Jakość

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected NSD1 on 10 µg of PC12 cell lysate.

Opis wartości docelowych

~ 300 kDa

Postać fizyczna

Unpurified

Unpurified mouse monoclonal IgG1κ in preservative-free ascites.

Przechowywanie i stabilność

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Komentarz do analizy

Control
PC12 cell lysate

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable