SRE0095

Fast β-Glucuronidase, Recombinant

from limpets (Patella Vulgata), aqueous solution

• Synonim(y): β-Glucuronidase from limpets (Patella vulgata), β-D-Glucuronide glucuronosohydrolase
• Numer CAS: 9001-45-0
• Numer EC enzymu: 3.2.1.31 (BRENDA, IUBMB)
• Numer MDL: MFCD00130629
• Kod UNSPSC: 12352200

Właściwości

• rekombinowane: expressed in proprietary host
• Poziom jakości: 200
• Postać: aqueous solution
• stężenie: 300000-400000 units/mL
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Zastosowanie

New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page.

The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Cechy i korzyści

Formulated with a higher enzyme concentration for applications requiring ultra-fast hydrolysis rates. Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→morphine). Representative analysis at high enzyme titer (50 un/μL β-glucuronidase) was less than 1% conversion of 6-MAM to morphine after 4 hours at 60 °C.

Definicja jednostki

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Postać fizyczna

Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable