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D8418

Sigma-Aldrich

Dimethyl sulfoxide

for molecular biology

Synonim(y):

Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO

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About This Item

Wzór liniowy:
(CH3)2SO
Numer CAS:
67-68-5
Masa cząsteczkowa:
78.13
Beilstein:
506008
Numer WE:
200-664-3
Numer MDL:
MFCD00002089
Kod UNSPSC:
12191502
Identyfikator substancji w PubChem:
24894214
NACRES:
NA.52

klasa czystości

for molecular biology

Poziom jakości

200

gęstość pary

2.7 (vs air)

ciśnienie pary

0.42 mmHg ( 20 °C)

linia produktu

BioReagent

Próba

≥99.9%

Postać

liquid

temp. samozapłonu

573 °F

okres trwałości

Recommended retest period - 2 years

granice wybuchowości

42 %, 63 °F

metody

DNA sequencing: suitable
PCR: suitable
transfection: suitable

zanieczyszczenia

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

kolor

colorless

współczynnik refrakcji

n20/D 1.479 (lit.)

tw

189 °C (lit.)

mp

16-19 °C (lit.)

rozpuszczalność

H2O: miscible (completely)

gęstość

1.10 g/mL (lit.)

absorpcja

passes test

przydatność

suitable for molecular biology

obecność zanieczyszczeń

DNase and RNase, none detected

temp. przechowywania

room temp

ciąg SMILES

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

Klucz InChI

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Opis ogólny

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Zastosowanie

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Cechy i korzyści

This product is free from DNases, RNases, phosphatases and proteases.

Przestroga

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

188.6 °F - closed cup

Temperatura zapłonu (°C)

87 °C - closed cup

Środki ochrony indywidualnej

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Advances in colloid and interface science, 222, 9-17 (2014-08-17)
The observation by Atomic Force Microscopy of a range of nanophases on hydrophobic surfaces poses some challenging questions, not only related to the stability of these objects but also regarding their wetting properties. Spherical capped nanobubbles are observed to exhibit
A Oguro-Ando et al.
Molecular psychiatry, 20(9), 1069-1078 (2014-10-15)
Rare maternally inherited duplications at 15q11-13 are observed in ~1% of individuals with an autism spectrum disorder (ASD), making it among the most common causes of ASD. 15q11-13 comprises a complex region, and as this copy number variation encompasses many
Caroline Godfrey et al.
Human molecular genetics, 24(15), 4225-4237 (2015-05-03)
Splice modulation therapy has shown great clinical promise in Duchenne muscular dystrophy, resulting in the production of dystrophin protein. Despite this, the relationship between restoring dystrophin to established dystrophic muscle and its ability to induce clinically relevant changes in muscle
Mulmudi Hemant Kumar et al.
Advanced materials (Deerfield Beach, Fla.), 26(41), 7122-7127 (2014-09-13)
Lead free perovskite solar cells based on a CsSnI3 light absorber with a spectral response from 950 nm is demonstrated. The high photocurrents noted in the system are a consequence of SnF2 addition which reduces defect concentrations and hence the
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