추천 제품
제품명
Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
생물학적 소스
Phaseolus limensis (lima bean)
유형
Type II-L
양식
crude powder
분자량
9 kDa
solubility
0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
저장 온도
2-8°C
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애플리케이션
Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:
- in the inhibition of trypsin in human plasma
- in the termination of proteolytic digestion in rabbit skeletal muscles
- in the inhibition of salivary glands enzyme extract from Lygus lineolaris
- in the inhibition of proteolytic activity in Agave tequilana enzyme extract
Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.
생화학적/생리학적 작용
Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.
물리적 특성
Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.
단위 정의
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.
제조 메모
Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).
분석 메모
One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.
기타 정보
View more information on Trypsin Inhibitor.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
G Bellon et al.
Artery, 7(4), 290-302 (1980-01-01)
A serine protease active on insoluble elastin at neutral pH has been isolated from human aortic media employing a Lima-bean trypsin inhibitor - Sepharose column. It is also hydrolyzed Suc (Ala)3 pna and casein but was found inactive against Benzoyl-Tyr-pna
In-house phase determination of the lima bean trypsin inhibitor: a low-resolution sulfur-SAD case
Debreczeni J, et al.
Acta crystallographica. Section D, Structural biology, 59(2), 393-395 (2003)
Are cardiovascular and sympathoadrenal effects of human ?new pressor protein? preparations attributable to human coagulation beta-FXIIa?
Papageorgiou PC, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 286(3), H837-H846 (2004)
A Rapid and Reliable Method for Total Protein Extraction from Succulent Plants for Proteomic Analysis
Lledias F, et al.
The Protein Journal, 36(4), 308-321 (2017)
Shark skeletal muscle tropomyosin is a phosphoprotein
Hayley M, et al.
Journal of Muscle Research and Cell Motility, 29(2-5), 101-107 (2008)
문서
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
프로토콜
Enzymatic Assay of Trypsin Inhibitor
Chromatograms
application for HPLC자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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