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Merck
모든 사진(5)

주요 문서

T2011

Sigma-Aldrich

Trypsin inhibitor

powder

동의어(들):

Ovomucoid

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.77

제품명

Trypsin inhibitor from chicken egg white, Type III-O (free of ovoinhibitor)

생물학적 소스

chicken egg white

Quality Level

유형

Type III-O (free of ovoinhibitor)

양식

powder

분자량

14 kDa

solubility

water: soluble 10 g/L

저장 온도

2-8°C

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관련 카테고리

일반 설명

Ovomucoid or trypsin inhibitor is an abundant protein in most avian egg whites. The hen′s egg protein is composed of about 186 amino acid residues, and is highly glycosylated. Three tandem domains are each homologous with pancreatic trypsin inhibitor. It is highly immunogenic, and probably accounts for most cases of egg allergy.

애플리케이션

Trypsin inhibitor from chicken egg white has been used:
  • to terminate proteolysis reaction
  • in OVO-1 solution to triturate the cell suspension for the dissociation of retina
  • in phosphate buffered saline for dissociation of cortices

생화학적/생리학적 작용

Ovomucoid possesses trypsin inhibitor activity. It has several conformational and linear epitopes that can be bound by immunoglobulin E (IgE). It is highly stable against heat and proteolysis.

단위 정의

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

기타 정보

View more information on Trypsin Inhibitor.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Meeyong Shin et al.
Allergy, asthma & immunology research, 5(1), 42-47 (2013-01-02)
It is known that ovomucoid, an egg allergen, is heat resistant and remains soluble after heating. However, a recent study showed that the antigenic activity of ovomucoid could be reduced by heating when egg white (EW) was mixed with wheat
ORF7 of Varicella-Zoster Virus Is Required for Viral Cytoplasmic Envelopment in Differentiated Neuronal Cells
Jiang HF, et al.
Journal of virology, 91(12) (2017)
Ovomucoid specific immunoglobulin E as a predictor of tolerance to cooked egg
Bartnikas LM, et al.
Allergy & rhinology (Providence, R.I.), 6(3), ar-2015 (2015)
Meeyong Shin et al.
Allergy, asthma & immunology research, 5(2), 96-101 (2013-03-02)
The present study was performed to determine the factor, either duration or the temperature of heat treatment, exerting maximal and significant influence on the composition and allergenicity of egg white (EW) proteins. Raw EW and 4 kinds of heated EW
Manipulation of the N-terminal sequence of the Borna disease virus X protein improves its mitochondrial targeting and neuroprotective potential
Ferree C, et al.
Faseb Journal (2015)

프로토콜

Enzymatic Assay of Trypsin Inhibitor

Chromatograms

application for HPLC

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