T2400

Tropomyosin from porcine muscle

lyophilized powder (contains Tris buffer salts)

• CAS Number: 9067-56-5
• MDL number: MFCD00132526
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: Porcine muscle
• Quality Level: 200
• 분석: ≥70% protein basis (biuret)
• 양식: lyophilized powder (contains Tris buffer salts)
• 기술: electrophoresis: suitable
• 저장 온도: −20°C

설명

일반 설명

Tropomyosin is a member of the actin filament binding protein family. It is a ubiquitous protein present in animals and fungi. Tropomyosin is made up of two α-helical chains arranged as a coiled-coil. It exists over twenty isoforms, in different eukaryotic cells like skeletal, cardiac, and smooth muscles, brain, platelets, fibroblasts, platelets, and other non-muscle cells.

애플리케이션

Tropomyosin from porcine muscle has been used:

• as a positive control in actin- α-tropomyosin (TPM1) co-sedimentation analysis and assay
• in analyzing the anti-adhesive properties of fish tropomyosins
• in far Western blot analysis

생화학적/생리학적 작용

Dimeric protein that assumes an α-helical coiled coil conformation. In the absence of Ca²⁺, inhibits muscle contractility by blocking the myosin binding sites on actin.

Tropomyosin is associated with the actin cytoskeleton and plays a role in stabilizing and regulating the interactions of actin with other actin-binding proteins. It also plays a role in muscle contraction by regulating the interactions of actin with myosin.

Tropomyosin together with troponin, regulates the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Various tropomyosin isoforms help confer structure and function to actins in the cytoskeleton and striated muscle function. Dimeric protein that assumes an α-helical coiled-coil conformation. In the absence of Ca2+, it inhibits muscle contractility by blocking the myosin binding sites on actin.

분석 메모

SDS electrophoresis shows two major bands.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)