추천 제품
생물학적 소스
bovine erythrocytes
제품 라인
BioReagent
형태
lyophilized powder
특이 활성도
≥3,000 units/mg protein
분자량
32.5 kDa
포장
pkg of 15000 units
기술
cell culture | mammalian: suitable
pH
7.6-10.5
배송 상태
dry ice
저장 온도
−20°C
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일반 설명
Superoxide Dismutase from bovine erythrocytes is a metalloprotein which disproportionates superoxide anion radicals. It is a 31.5 kDa copper binding protein and displays a conserved domain and fold. It is a homodimer with one copper and zinc ion per subunit and has antiparallel “greek-key” β barrel fold.
애플리케이션
Superoxide Dismutase (SOD) from bovine erythrocytes has been used:
- for measuring the superoxide radical using the electron paramagnetic resonance spin in human brain microvascular endothelial cells
- for measuring superoxide production in cytochrome C assay in peripheral blood mononuclear cells
- as a standard in characterization of hen egg SOD using Fourier-transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption/ionization (MALDI) analysis
생화학적/생리학적 작용
Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.
단위 정의
One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
분석 메모
For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Chunxia Xiao et al.
PloS one, 7(1), e30343-e30343 (2012-01-25)
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It is now well established that both inherited and acquired forms of hemolytic disease can promote pulmonary vascular disease consequent of free hemoglobin (Hb) induced NO scavenging, elevations in reactive oxygen species and lipid peroxidation. It has recently been reported
Partial biochemical characterization of Cu, Zn-superoxide dismutase extracted from eggs of hens (Gallus gallus domesticus)
Wawrzykowski J and Kankofer M
Food Chemistry, 227, 390-396 (2017)
human cystic fibrosis macrophages have defective calcium-dependent protein kinase C activation of the NADPH oxidase, an effect augmented by Burkholderia cenocepacia
Assani K, et al.
Journal of immunology (Baltimore, Md. : 1950), 198(5), 1985-1994 (2017)
Structure of fully reduced bovine copper zinc superoxide dismutase at 1.15
Hough MA and Hasnain SS
Structure, 11(8), 937-946 (2003)
프로토콜
Enzymatic Assay of Superoxide Dismutase
Separation of Superoxide dismutase
Chromatograms
application for HPLC자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.