S7571

Superoxide Dismutase from bovine erythrocytes

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

• 동의어(들): CU/ZN-SOD, Superoxide Dismutase 1 bovine, cytocuprein, erythrocuprein, hemocuprein, SOD, Superoxide: superoxide oxidoreductase
• CAS Number: 9054-89-1
• 효소 위원회 번호: 1.15.1.1 (BRENDA, IUBMB)
• EC Number: 232-943-0
• MDL number: MFCD00132404
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine
• 형태: lyophilized powder
• 특이 활성도: ≥3,000 units/mg protein
• 분자량: 32.5 kDa
• 구성: Protein, ≥95% biuret
• 저장 조건: (Store under nitrogen.; Tightly closed. Dry.)
• 환경친화적 대안 제품 특성: Atom Economy; Design for Energy Efficiency; Use of Renewable Feedstocks; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 기술: immunoblotting: suitable; inhibition assay: suitable
• 색상: blue-green
• pI: 4.95
• solubility: water: 20 mg/mL; aqueous buffer, pH 7.5: soluble
• UniProt 수납 번호: P00442; P41976
• 응용 분야: diagnostic assay manufacturing
• 환경친화적 대안 카테고리: , Re-engineered
• 저장 온도: −20°C
• 유전자 정보: cow ... SOD1(281495) , SOD2(281496)

설명

일반 설명

Research area: Cell Signaling

Superoxide dismutase (SOD) is a redox-active metalloenzyme expressed in both aerobic and anaerobic living organisms. Bovine superoxide dismutase or CuZn SOD is a homodimer with each subunit containing one zinc and one copper ion.

애플리케이션

Superoxide dismutase from bovine erythrocytes has been used:

• in a study to assess a kinetic model of radiation-induced inactivation of superoxide dismutase in nitrous oxide-saturated solutions
• in a study to investigate the possible participation of superoxide anion in the intestinal tryptophan 2,3-dioxygenase reaction
• to investigate its effect on the hemolysis rate of human RBCs and hemoglobin-nitric oxide complex (HbNO) stability in human erythrocytes
• in combination with catalase to study its effect on cell differentiation in vitro
• to quantify superoxide levels and study their effect on reactivity in mouse pulmonary arteries through chemiluminescence and cytochrome C reduction methods

생화학적/생리학적 작용

Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

단위 정의

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 ml reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

물리적 형태

Lyophilized powder, essentially salt-free

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)