T9777

Trypsin-chymotrypsin inhibitor

lyophilized powder

• 동의어(들): Bowman-Birk Inhibitor
• CAS Number: 37330-34-0
• MDL number: MFCD00132532
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• product name: Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder
• 생물학적 소스: Glycine max (soybean)
• Quality Level: 200
• 형태: lyophilized powder
• 구성: Protein, 70-90% biuret
• 저장 온도: 2-8°C

설명

일반 설명

Trypsin-chymotrypsin inhibitor belongs to the family of plant protease inhibitors. It is mainly found in the legume (Fabaceae) and cereal (Poaceae) families. Trypsin-chymotrypsin inhibitor consists of many internal disulfide bonds between conserved Cys residues, which maintains structural stability and active conformation of the inhibitory loops. Trypsin-chymotrypsin has anticarcinogenic properties.

Highly cross-linked with seven disulfide bridges, Bowman-Birk inhibitor has spatially distinct domains for trypsin and chymotrypsin inhibition.

애플리케이션

Bowman Birk protease inhibitor prevents radiation-induced carcinogenesis by a reduction of incorrect DNA repairs, resulting in a reduced amount of dicentric chromosomes.

Trypsin-chymotrypsin inhibitor from Glycine max (soybean) has been used for flow cytometric separase activity assay. It has also been used to inhibit trypsin and chymotrypsin for in vitro gastrointestinal (GI) digestion studies.

단위 정의

One trypsin unit = ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 mL (1 cm light path).

물리적 형태

Lyophilized powder containing phosphate buffer salts, pH 7.6

분석 메모

1 mg protein will inhibit ≥0.5 mg trypsin with activity of ~10,000 BAEE units per mg protein or ≥1.0 mg chymotrypsin with activity of ~40 BTEE units per mg protein.

기타 정보

View more information on Trypsin Inhibitor.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)