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Merck
모든 사진(1)

주요 문서

SRP3133

Sigma-Aldrich

OX40 Ligand human

recombinant, expressed in Hi-5 Insect cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture

동의어(들):

CD252, Gp34, OX40L, TNFSF4

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.32

생물학적 소스

human

재조합

expressed in Hi-5 Insect cells

분석

≥97% (HPLC)
≥97% (SDS-PAGE)

양식

lyophilized

분자량

15.5-25.0 kDa

포장

pkg of 10 μg

기술

cell culture | mammalian: suitable

불순물

<0.1 EU/μg endotoxin, tested

색상

white to off-white

UniProt 수납 번호

배송 상태

wet ice

저장 온도

−20°C

유전자 정보

human ... TNFSF4(7292)

일반 설명

OX40L or tumor necrosis factor superfamily member 4 (TNFSF4), which is a member of the TNF superfamily of structurally related proteins, exists primarily as a type II membrane bound, non-covalently linked homotrimeric protein. This cytokine is expressed on antigen presenting cells (APCs), such as dendritic cells and activated B-cells, and also on various other cells such as vascular endothelial cells, mast cells, and natural killer cells. It has a 23 amino acid cytoplasmic tail and a 133 amino acid extracellular domain. The gene encoding this protein is localized on human chromosome 1q25 and has three exons. Recombinant OX40L is a glycosylated 133 amino acid protein corresponding to the extracellular TNF homologous domain of the full length transmembrane protein. It migrates with an apparent molecular mass of 15.5-25kDa on SDS-PAGE.

생화학적/생리학적 작용

OX40L signals specifically through the OX40 receptor, which is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. OX40/OX40L functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of regulated on activation, normal T cell expressed and secreted (RANTES) or chemokine ligand 5 (CCL5), interleukins-2 and -3, and interferon-γ (IFNγ). OX40/OX40L signaling plays an important role in immuno-regulatory communication and immune-tolerance. Polymorphisms in the TNFSF4 gene have been associated with autoimmune thyroid diseases (AITDs), rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc).

서열

QVSHRYPRIQ SIKVQFTEYK KEKGFILTSQ KEDEIMKVQN NSVIINCDGF YLISLKGYFS QEVNISLHYQ KDEEPLFQLK KVRSVNSLMV ASLTYKDKVY LNVTTDNTSL DDFHVNGGEL ILIHQNPGEF CVL

물리적 형태

Lyophilized from 10 mM Tris pH 8.5.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

A diametric role for OX40 in the response of effector/memory CD4+ T cells and regulatory T cells to alloantigen
Gillain Kinnear
Journal of Immunology, 191(3), 1465-1475 (2013)
Control of Immunity by the TNFR-Related Molecule OX40 (CD134)
Michael Croft
Annual Review of Immunology, 28, 57-78 (2010)
TNFSF4 Gene Variations Are Related to Early-Onset Autoimmune Thyroid Diseases and Hypothyroidism of Hashimoto?s Thyroiditis
Rong-Hua Song
International Journal of Molecular Sciences, 17(8), 1369-1369 (2016)
Deborah S Cunninghame Graham et al.
Nature genetics, 40(1), 83-89 (2007-12-07)
Systemic lupus erythematosus (SLE) is a multisystem complex autoimmune disease of uncertain etiology (OMIM 152700). Over recent years a genetic component to SLE susceptibility has been established. Recent successes with association studies in SLE have identified genes including IRF5 (refs.
Qigui Yu et al.
Journal of immunology (Baltimore, Md. : 1950), 176(4), 2486-2495 (2006-02-04)
We have previously shown that CD4(+) T cells are required to optimally expand viral-specific memory CD8(+) CTL responses using a human dendritic cell-T cell-based coculture system. OX40 (CD134), a 50-kDa transmembrane protein of the TNFR family, is expressed primarily on

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