SRP0235

PI3 kinase [p110a(H1047R)/p85a] Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

• 동의어(들): PIK3CA, Phosphoinositide-3-kinase, catalytic α, PtdIns-3-kinase p110
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in baculovirus infected insect cells
• 분석: ≥60% (SDS-PAGE)
• 형태: aqueous solution
• 특이 활성도: ≥1770 pmol/min-μg
• 분자량: 129 kDa (p110α); 88 kDa (p85α)
• 포장: pkg of 20 μg
• 농도: 0.38 mg/mL
• NCBI 수납 번호: U79143 (p110alpha); XM_043865 (p85alpha)
• UniProt 수납 번호: P42336
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PIK3CA(5290)

설명

일반 설명

Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. U79143), with H1047R mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.

애플리케이션

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

단위 정의

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2