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Merck
모든 사진(2)

Key Documents

SRP0233

Sigma-Aldrich

PI3 kinase (p110a/p85a) Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

동의어(들):

PI3-kinase regulatory subunit 4, PIK3R4, Phosphoinositide 3-kinase adaptor protein, VPS15, PIK3CA, Phosphoinositide-3-kinase, catalytic, alpha, PtdIns-3-kinase p110

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.32

생물학적 소스

human

재조합

expressed in baculovirus infected insect cells

분석

≥80% (SDS-PAGE)

형태

aqueous solution

특이 활성도

≥13 pmol/min-μg

분자량

129 kDa (p110α)
88 kDa (p85α)

포장

pkg of 20 μg

제조업체/상표

Sigma-Aldrich

농도

>0.02 mg/mL

기술

activity assay: suitable

UniProt 수납 번호

응용 분야

life science and biopharma

배송 상태

dry ice

저장 온도

−70°C

유전자 정보

human ... PIK3CA(5290)

일반 설명

Research area: Cell Signaling

PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit a) is one of the four subunits of the 110kDa catalytic subunit of PI3K protein, which contains a regulatory region of 85kDa. PI3K proteins form a family of lipid kinases. This gene is localized to human chromosome 3q.Three different classes of PI3Ks (I – III) have been discovered.

애플리케이션

PI3 kinase (p110a/p85a) Active human has been used for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

생화학적/생리학적 작용

PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit a) is one of the four catalytic subunits of PI3K protein, which plays an essential role in extracellular growth signaling. It phosphorylates and activates protein kinase B (PKB) protein. This protein has a wide range of physiological functions, such as proliferation, cell cycle, survival, adhesion, motility, apoptosis, angiogenesis, DNA repair, senescence, and cellular metabolism, and plays a central role in multiple types of cancers. It also functions as an intermediary in cellular signaling and is primarily recognized for its involvement in the Phosphoinositide 3-kinase/ Protein kinase B/ mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway. PIK3CA gene is either mutated or amplified in brain, breast, stomach, liver, ovary, and colon cancers. Mutation in this gene leads to enhanced PI3K activity, and it is a candidate transforming oncogene. It is over-expressed in invasive breast cancer and is a marker for poor prognosis. This gene is amplified in high-grade dysplastic lesions from bronchial biopsy specimens obtained from preinvasive squamous lung cancer. Frequency of mutations in the PIK3CA gene is higher in tumors without lymph node metastasis than in those with gastric cancer. Thus, it might be essential to evaluate the mutations in this gene to determine cetuximab treatment in gastric cancer patients.

단위 정의

One unit of 110a/p85a kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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문서 라이브러리 방문

PIK3CA expression in invasive breast cancer: a biomarker of poor prognosis.
Aleskandarany MA, et al.
Breast Cancer Research and Treatment, 122(1), 45-53 (2010)
Progressive 3q amplification consistently targets SOX2 in preinvasive squamous lung cancer.
McCaughan F, et al.
American Journal of Respiratory and Critical Care Medicine, 182(1), 83-91 (2010)
Weipeng Lu et al.
Molecular medicine reports, 12(1), 1219-1224 (2015-03-31)
Cetuximab, an immunoglobulin G1 chimeric monoclonal antibody directed against the epidermal growth factor receptor, is currently considered to be the strategy with the most potential for the treatment of gastric cancer due to the low frequency of KRAS mutations in

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