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Merck
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Key Documents

S6402

Sigma-Aldrich

Streptavidin−Cy3 from Streptomyces avidinii

buffered aqueous solution

동의어(들):

Streptavidin Cy3

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

결합

CY3 conjugate

Quality Level

형태

buffered aqueous solution

라벨링 범위

3-9 mol fluorochrome per mol protein

기술

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

Streptavidin is a homotetramer protein. One molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin has a high affinity for biotin and is extensively used as a labeling tool and for studies involving binding proteins.

애플리케이션

Streptavidin−Cy3 from Streptomyces avidinii has been used:
  • in double immunofluorescence staining to detect biotinylated lectin
  • for insulin and C-peptide staining in immunofluorescence analysis
  • in double immunofluorescence to verify the presence of cells filled with biocytin
  • in conjunction with biotinylated antibodies in immunohistochemistry and immunocytochemistry
  • with the traditional rhodamine filter arrangements due to the spectral overlap

특징 및 장점

Spectral Characteristics of Cy3 Dye:
  • Absorbance Max 552 nm
  • Emission Max 565 nm
  • Molar Extinction Coefficient 1.3 x 10-5 M-1cm-1

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

법적 정보

Cy3 is a trademark of Cytiva

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

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