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Merck
모든 사진(2)

주요 문서

85878

Sigma-Aldrich

Streptavidin from Streptomyces avidinii

affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.32

양식

powder

Quality Level

품질

affinity purified
lyophilized from 10 mM potassium phosphate

특이 활성도

≥13 U/mg protein

분자량

Mr ~60000

solubility

H2O: 1 mg/mL, clear to hazy, colorless to faintly yellow

저장 온도

−20°C

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일반 설명

Streptavidin is a crystalline protein synthesized by Streptomycetes.

애플리케이션

Streptavidin from Streptomyces avidinii has been used:
  • in the pre-functionalization of self-propelled catalytic micromotors
  • in the fabrication and modification of hydroxyapatite-chitosan (HA-CTS) nanofilm-coated substrates
  • as an analyte to bind biomolecules to the gold-inverted polymer solar cells (Au-IPSC)

생화학적/생리학적 작용

Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.

포장

Bottomless glass bottle. Contents are inside inserted fused cone.
The sales quantitites 1 mg and 5 mg

단위 정의

1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5

분석 메모

Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied.

기타 정보

Review: Application of the avidin-biotin technique in microbiology; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin; Avidin-Biotin technology

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

D.A. Fuccillo
Biotechniques, 3, 494-494 (1985)
Biofunctionalized self-propelled micromotors as an alternative on-chip concentrating system
Restrepo-Perez L, et al.
Lab on a chip, 14(16), 2914-2917 (2014)
Capture and identification of heterogeneous circulating tumor cells using transparent nanomaterials and quantum dots-based multiplexed imaging
Chen Y Y, et al.
Journal of Cancer, 7(1), 69-69 (2016)
Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding
Magalhaes M L B, et al.
Protein Science, 20(7), 1145-1154 (2011)
Soon Gang Choi et al.
Nature communications, 10(1), 3907-3907 (2019-08-31)
Complementary assays are required to comprehensively map complex biological entities such as genomes, proteomes and interactome networks. However, how various assays can be optimally combined to approach completeness while maintaining high precision often remains unclear. Here, we propose a framework

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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