추천 제품
형태
liquid
사용
sufficient for 50 reactions
특징
Long & Accurate PCR
dNTPs included: no
hotstart: no
농도
2.5 units/μL
기술
PCR: suitable
색상
colorless
입력
purified DNA
배송 상태
wet ice
저장 온도
−20°C
일반 설명
Restorase DNA Polymerase with 10× Reaction Buffer combines Sigma′s long and accurate enzyme technology with a small amount of DNA repair enzyme. The optimized blend will initiate the repair and further amplification of damaged DNA templates greater than 800 bp. Restorase has also been shown to increase yield on undamaged DNA templates.
애플리케이션
Restorase® DNA Polymerase with 10× Reaction Buffer has been used in DNA repair.
생화학적/생리학적 작용
DNA templates are often damaged on exposure to heat, acids, alkylating agents, and light. These damaged templates result in inefficient or failed DNA amplification by polymerase chain reaction (PCR). Restorase® DNA Polymerase repairs the damaged sites on the DNA templates and initiates subsequent template copying. Restorase treatment of the DNA depends on the level of template damage.
특징 및 장점
- Reliable amplification of damaged DNA
- When thermostable polymerases fail, this enzyme amplifies the sequence efficiently
- Efficient amplification of sequences in multiplex reactions
- Increased yield and amplicon specificity
- Amplifies a broad spectrum of amplicon sizes varying from 200 bp to 20 kb
- Can repair 3′ bungs, nicks, and abasic sites
포장
The enzyme is provided with an optimized 10× reaction buffer provided as 1 vial/250 units.
기타 정보
Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.
View more detailed information on Restorase DNA Polymerase at www.sigma-aldrich.com/restorase.
법적 정보
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Restorase is a registered trademark of Merck KGaA, Darmstadt, Germany
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Francesca Zinetti et al.
PloS one, 8(6), e65746-e65746 (2013-06-12)
There is increasing evidence that most parapatric cryptic/sister taxa are reproductively compatible across their areas of contact. Consequently, the biological species concept, which assumes absence of interbreeding, is becoming a not so effective criterion in evolutionary ecology. Nevertheless, the few
Mehrdad Hajibabaei et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 360(1462), 1959-1967 (2005-10-11)
Large-scale DNA barcoding projects are now moving toward activation while the creation of a comprehensive barcode library for eukaryotes will ultimately require the acquisition of some 100 million barcodes. To satisfy this need, analytical facilities must adopt protocols that can
Innis, M.A., et al.
PCR Protocols: A Guide to Methods and Applications (1990)
James M Robertson et al.
Forensic science international. Genetics, 12, 168-180 (2014-07-06)
Forensic scientists have used several approaches to obtain short tandem repeat (STR) profiles from compromised DNA samples, including supplementing the polymerase chain reaction (PCR) with enhancers and using procedures yielding reduced-length amplicons. For degraded DNA, the peak intensities of the
Shigeo Yagi et al.
Parasitology research, 102(2), 211-217 (2007-09-28)
Granulomatous amoebic encephalitis (GAE) is a usually fatal disease caused by the free-living amoebae Balamuthia mandrillaris and Acanthamoeba spp. The intent of this study was to determine if the polymerase chain reaction (PCR) could be used retrospectively to detect amoeba
문서
Restorase® DNA Polymerase
Restorase® was developed for researchers unable to achieve amplification of damaged DNA templates when using other commercially available DNA polymerases.
프로토콜
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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