P6516

Poly-L-lysine hydrobromide

suitable for cell culture, Mol wt ≤15,000 by MALLS

• 동의어(들): L-Lysine homopolymer hydrobromide
• CAS Number: 25988-63-0
• MDL number: MFCD00237230
• UNSPSC 코드: 12352209
• PubChem Substance ID: 24898773
• NACRES: NA.26

속성

• product name: Poly-L-lysine hydrobromide, mol wt 4,000-15,000 by viscosity
• 형태: solid
• 분자량: ≤15,000 by MALLS; 4,000-15,000 by viscosity
• 환경친화적 대안 제품 점수: old score: 51; new score: 43; Find out more about DOZN™ Scoring
• 환경친화적 대안 제품 특성: Designing Safer Chemicals; Safer Solvents and Auxiliaries; Reduce Derivatives; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 기술: cell culture | mammalian: suitable
• 색상: white to off-white
• 응용 분야: cell analysis
• 환경친화적 대안 카테고리: , Re-engineered
• 저장 온도: −20°C
• SMILES string: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• InChI key: WBSCNDJQPKSPII-KKUMJFAQSA-N

설명

일반 설명

We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Designing Safer Chemicals”, "Safer Solvents and Auxiliaries" and “Reduce Derivatives”. Click here to view its DOZN scorecard.

애플리케이션

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule. Poly-L-lysine hydrobromide has been used:

• as a component of polyplexes and in DNA condensation experiments
• for coating circular mica disks for atomic force microscopy studies and SuperFrost glass slides for immunofluorescence studies of in HL-60 cells
• as a reference standard for generating calibration curve for the quantification of ε- Poly-L-lysine

생화학적/생리학적 작용

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

성분

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

주의사항

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

제조 메모

This product has a molecular weight of 4,000-15,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)